Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo, Toyo 113-0033, Japan.
J Biophotonics. 2012 Jan;5(1):20-4. doi: 10.1002/jbio.201100087. Epub 2011 Nov 11.
We observe the redox state changes with respiration of cytochromes b and c in mitochondria in a living Saccharomyces cerevisiae cell as well as in isolated mitochondria with the very use of Raman microspectroscopy. The possibility of monitoring the respiration activity of mitochondria in vivo and in vitro by Raman microspectroscopic quantification of the cytochrome redox states is suggested. It will lead to a new means to assess mitochondrial respiration activity in vivo and in vitro without using any labelling or genetic manipulation.
我们观察到活酿酒酵母细胞和分离的线粒体中细胞色素 b 和 c 的呼吸作用引起的氧化还原状态变化，这是通过拉曼微光谱技术非常实现的。通过拉曼微光谱定量细胞色素的氧化还原状态，提示了监测体内和体外线粒体呼吸活性的可能性。这将为评估体内和体外线粒体呼吸活性提供一种新的手段，而无需使用任何标记或遗传操作。
