Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
C R Biol. 2011 Nov;334(11):795-805. doi: 10.1016/j.crvi.2011.06.004. Epub 2011 Aug 25.
An exhaustive screening of the Pleurotus ostreatus genome was performed to search for nucleotide sequences of heme peroxidases in this white-rot fungus, which could be useful for different biotechnological applications. After sequence identification and manual curation of the corresponding genes and cDNAs, the deduced amino acid sequences were converted into structural homology models. A comparative study of these sequences and their structural models with those of known fungal peroxidases revealed the complete inventory of heme peroxidases of this fungus. This consists of cytochrome c peroxidase and ligninolytic peroxidases, including manganese peroxidase and versatile peroxidase but not lignin peroxidase, as representative of the "classical" superfamily of plant, fungal, and bacterial peroxidases; and members of two relatively "new" peroxidase superfamilies, namely heme-thiolate peroxidases, here described for the first time in a fungus from the genus Pleurotus, and dye-decolorizing peroxidases, already known in P. ostreatus but still to be thoroughly explored and characterized.
对糙皮侧耳（Pleurotus ostreatus）基因组进行了全面筛选，以寻找该白腐真菌中血红素过氧化物酶的核苷酸序列，这些序列可用于不同的生物技术应用。在对相应的基因和 cDNA 进行序列鉴定和人工注释后，将推导出的氨基酸序列转化为结构同源模型。对这些序列及其与已知真菌过氧化物酶的结构模型进行比较研究，揭示了该真菌血红素过氧化物酶的完整清单。这包括细胞色素 c 过氧化物酶和木质素降解过氧化物酶，包括锰过氧化物酶和多功能过氧化物酶，但不包括木质素过氧化物酶，它们是植物、真菌和细菌过氧化物酶“经典”超家族的代表；以及两个相对“新”的过氧化物酶超家族的成员，即血红素硫醇过氧化物酶，本文首次在糙皮侧耳属的真菌中进行了描述，以及染料脱色过氧化物酶，已经在糙皮侧耳（P. ostreatus）中被发现，但仍需要深入探索和表征。
