The University of Iowa, College of Dentistry, Dows Institute for Dental Research, 801 Newton Road, Iowa City, IA 52242-1010, USA.
Dent Mater. 2012 Mar;28(3):252-60. doi: 10.1016/j.dental.2011.10.010. Epub 2011 Nov 9.
Our goal was to characterize the response of human mesenchymal stem cells (hMSCs) to a niobium-doped fluorapatite-based glass-ceramic (FAp).
The glass was prepared by twice melting at 1525 °C for 3 h, and cast into cylindrical ingots later sectioned into discs and heat-treated to promote crystallization of fluorapatite submicrometer crystals. Tissue culture polystyrene (TCP) was used as control. The surface of the FAp discs was either left as-heat treated, ground or etched. Initial cell attachment was assessed at 3 h. Proliferation and alkaline phosphatase (ALP) expression data were collected at days 1, 4, and 8. Cell morphology was examined using SEM, at days 2 and 4. Mineralization was evaluated by Alizarin Red staining and SEM.
Initial cell attachment on as heat-treated, etched, or ground surfaces was similar to that of the positive control group (p>0.05). The percentage of area covered by living cells increased significantly on as heat-treated, etched, or ground surfaces between days 1 and 8 (p<0.05). There was no significant difference among groups in cell coverage at day 8, compared to TCP control. SEM revealed well spread polygonal cells with numerous filopodia, either attached to the ceramic surface or connected to neighboring cells. ALP expression at day 8 was significantly higher in osteogenic media compared to growth media on both FAp and control. FAp discs stained positively with Alizarin Red and calcium-rich mineralized granules associated with fibrils were observed by SEM at day 35.
hMSCs displayed excellent attachment, proliferation, and differentiation on niobium-doped FAp glass-ceramic.
本研究旨在探讨人骨髓间充质干细胞（hMSCs）对掺铌氟磷灰石基玻璃陶瓷（FAp）的反应特征。
玻璃采用两次 1525°C 熔融 3 h 制备，随后浇铸成圆柱形铸锭，再将其切割成圆盘并进行热处理以促进氟磷灰石亚微米晶体的结晶。组织培养聚苯乙烯（TCP）用作对照。FAp 圆盘的表面状态为热处理状态、研磨状态或酸蚀状态。在 3 h 时评估初始细胞黏附。在第 1、4 和 8 天收集增殖和碱性磷酸酶（ALP）表达数据。在第 2 和 4 天使用 SEM 检查细胞形态。通过茜素红染色和 SEM 评估矿化情况。
在热处理、酸蚀或研磨表面上，初始细胞黏附与阳性对照组相似（p>0.05）。在第 1 天至第 8 天之间，热处理、酸蚀或研磨表面上活细胞覆盖的百分比显著增加（p<0.05）。与 TCP 对照组相比，第 8 天各组的细胞覆盖率无显著差异。SEM 显示出大量具有丝状伪足的多边形细胞，这些细胞附着在陶瓷表面或与相邻细胞相连。与生长培养基相比，在成骨培养基中，第 8 天 ALP 的表达在 FAp 和对照组中均显著升高。FAp 圆盘在第 35 天用茜素红染色呈阳性，并且 SEM 观察到与纤维相关的富含钙的矿化颗粒。
hMSCs 在掺铌氟磷灰石基玻璃陶瓷上表现出良好的黏附、增殖和分化。
