Dhevi G R Ishwarya, Arangannal Ponnudurai, Jeevarathan J
Department of Pedodontics and Preventive Dentistry, Sree Balaji Dental College and Hospital, Chennai, Tamil Nadu, India.
J Pharm Bioallied Sci. 2024 Dec;16(Suppl 4):S3951-S3955. doi: 10.4103/jpbs.jpbs_1078_24. Epub 2024 Dec 27.
Bioceramic materials have revolutionized the field of endodontics by successfully transforming the outcomes of pulp therapies. Novel biomaterials are evolving by modifying the conventional mineral trioxide aggregate (MTA) to overcome their existing limitations, the major ones being prolonged setting and cytotoxic radiopacifiers. Dental white portland cement (DWPC) is a novel formulated bioceramic material introduced as an enhanced MTA alternative. This in vitro study aims to investigate the cytotoxicity and odontogenic differentiation of novel formulation DWPC with MTA Angelus, Biodentin (BD) and white portland cement (WPC) on human dental pulp stem cells (HDPSCs).
HDPSCs were cultured in Dulbecco's modified eagle's medium (DMEM) with fetal bovine serum (FBS) and antibiotics at 37°C, 90% humidity in a 5% CO incubator. Experimental samples were prepared as disks. The viability of HDPSCs was measured by Mosmann's tetrazolium toxicity (MTT) assay, and odontogenic potential was assessed using alkaline phosphatase (ALP) activity at 24 hours, 7-, 14-, and 21-day intervals. The mean and standard deviations were statistically analyzed by one-way ANOVA and Scheffe tests using IBM SPSS Software (IBM statistical package for social sciences) - Version 24.0 with a significance level set as < 0.05.
All four groups tested using MTT assay showed no toxicity and possess odontogenic potential in all the experimental durations. Experimental group DWPC presented with the highest mean cell viability and ALP activity at all intervals followed by WPC, MTA, and BD ( < 0.05).
DWPC presented good bioactivity in terms of cell viability and ALP activity. Thus, DWPC could be a promising endodontic material. However, further research is warranted to explore the clinical applicability.
生物陶瓷材料通过成功改变牙髓治疗的结果，给牙髓病学领域带来了变革。新型生物材料通过改良传统的三氧化矿物凝聚体（MTA）不断发展，以克服其现有局限性，主要局限性在于凝固时间长和具有细胞毒性的射线阻射剂。牙科白色波特兰水泥（DWPC）是一种新配制的生物陶瓷材料，作为改良MTA的替代品被引入。本体外研究旨在探讨新型配方的DWPC与MTA Angelus、生物牙本质（BD）和白色波特兰水泥（WPC）对人牙髓干细胞（HDPSCs）的细胞毒性和牙源性分化。
HDPSCs在含有胎牛血清（FBS）和抗生素的杜氏改良 Eagle 培养基（DMEM）中，于37°C、湿度90%的5%二氧化碳培养箱中培养。将实验样品制备成圆盘状。通过Mosmann四唑盐毒性（MTT）试验测定HDPSCs的活力，并在24小时、7天、14天和21天的时间间隔使用碱性磷酸酶（ALP）活性评估其牙源性潜力。使用IBM SPSS软件（社会科学统计软件包）-版本24.0，通过单因素方差分析和Scheffe检验对平均值和标准差进行统计分析，显著性水平设定为<0.05。
使用MTT试验测试的所有四组在所有实验时间段均未显示出毒性，并且具有牙源性潜力。实验组DWPC在所有时间间隔均呈现出最高的平均细胞活力和ALP活性，其次是WPC、MTA和BD（<0.05）。
DWPC在细胞活力和ALP活性方面表现出良好的生物活性。因此，DWPC可能是一种有前途的牙髓治疗材料。然而，有必要进一步研究以探索其临床适用性。
