Department of Bionano Engineering, Hanyang University, Ansan 426-791, South Korea.
Nanoscale. 2012 Jan 7;4(1):124-9. doi: 10.1039/c1nr11243k. Epub 2011 Nov 11.
We report a highly sensitive optical imaging technology using surface-enhanced Raman scattering (SERS)-fluorescence dual modal nanoprobes (DMNPs). Fluorescence microscopy is a well-known imaging technique that shows specific protein distributions within cells. However, most currently available fluorescent organic dyes have relatively weak emission intensities and are rapidly photo-bleached. Thus more sensitive and stable probes are needed. In this work we develop DMNPs, which can be used for both SERS and fluorescence detection. SERS detection is a powerful technique that allows ultrasensitive chemical or biochemical analysis through unlimited multiplexing and single molecule sensitivity. Combining advantages of fluorescence and SERS allows these dual modal nanostructures to be used as powerful probes for novel biomedical imaging. In this work, the fabrication and characterization of the SERS-fluorescence DMNPs and application to biological imaging were investigated using markers CD24 and CD44, which are co-expressed in MDA-MB-231 breast cancer cells, as a model system. SERS imaging with DMNPs was found to be a powerful tool to determine the co-localization of CD24 and CD44 in the cell.
我们报告了一种使用表面增强拉曼散射（SERS）-荧光双模纳米探针（DMNP）的高灵敏度光学成像技术。荧光显微镜是一种众所周知的成像技术，可显示细胞内特定蛋白质的分布。然而，目前大多数可用的荧光有机染料的发射强度相对较弱，并且容易光漂白。因此，需要更灵敏和稳定的探针。在这项工作中，我们开发了 DMNP，它可用于 SERS 和荧光检测。SERS 检测是一种强大的技术，通过无限的多路复用和单分子灵敏度，允许对超灵敏的化学或生物化学分析。荧光和 SERS 的结合优势使这些双模纳米结构能够用作新型生物医学成像的强大探针。在这项工作中，使用共表达于 MDA-MB-231 乳腺癌细胞的 CD24 和 CD44 作为模型系统，研究了 SERS-荧光 DMNP 的制备和表征及其在生物成像中的应用。发现 DMNP 的 SERS 成像可有力地确定细胞中 CD24 和 CD44 的共定位。
