Faculty of Veterinary Science, The University of Melbourne, Melbourne, Vic., Australia.
Parasite Immunol. 2013 Feb;35(2):99-102. doi: 10.1111/pim.12009.
Cystic echinococcosis caused by Echinococcus granulosus remains an important and neglected issue in public health. The study of the likely efficacy of the currently available EG95 vaccine against other genotypes of the parasite is important to improve the vaccine as a potential tool to be used in control programmes. The recombinant vaccine EG95-1G1 was developed based on the G1 genotype of E. granulosus. Characterization of the eg95 gene family in the G6 genotype by genomic DNA cloning previously produced the first unequivocal information about the composition of the gene family in a different genotype. The information was used in this study to predict and express two EG95-related proteins from the G6 genotype as recombinants, for assessment of their capacity to bind antibodies raised in sheep vaccinated with the EG95-1G1 vaccine. The proteins (EG95-1G6 and EG95-5G6) from the G6 genotype of E. granulosus were unable to bind all the antibodies raised by sheep vaccinated with EG95-1G1. Differences in the amino acid sequence of EG95-related proteins from G6 and likely the differences in the encoded FnIII domain may be responsible for changes in the conformation of these epitopes.
由细粒棘球绦虫引起的包虫病仍然是公共卫生领域一个重要且被忽视的问题。研究目前可用的 EG95 疫苗对寄生虫其他基因型的可能疗效，对于改进该疫苗作为控制规划中潜在工具非常重要。重组疫苗 EG95-1G1 是基于细粒棘球绦虫的 G1 基因型开发的。通过基因组 DNA 克隆对 G6 基因型的 eg95 基因家族进行表征，以前提供了有关不同基因型中基因家族组成的第一个明确信息。该信息用于本研究中，以预测和表达来自 G6 基因型的两种与 EG95 相关的蛋白质作为重组体，以评估它们与用 EG95-1G1 疫苗接种的绵羊产生的抗体结合的能力。来自细粒棘球绦虫 G6 基因型的蛋白质（EG95-1G6 和 EG95-5G6）无法结合用 EG95-1G1 疫苗接种的绵羊产生的所有抗体。EG95 相关蛋白的氨基酸序列差异，可能还有编码的 FnIII 结构域的差异，可能导致这些表位构象发生变化。
