Delahunty T, Hollander D
Clin Chem. 1986 Feb;32(2):351-3.
The various Mr fractions of polyethylene glycol (PEG) in human urine are quantified by "high-pressure" liquid chromatography. A simple preparation step involving lyophilization and chloroform extraction of the sample is required. Aliquots (10 microL) are chromatographed isocratically in equivolume mixtures of methanol and water on a column of styrene divinylbenzene and the refractive index of the effluent is measured. The results vary linearly with the concentrations of standards up to at least 10 g/L, and the six major fractions are clearly identifiable in injected samples containing 2 g or more of total PEG per liter. As little as 4 g/L can be precisely quantified, but for assessing the individual fractions, we recommend a minimum sample concentration of 7 g/L. Analytical recovery of PEG added to urine controls was 90%. Urines collected during 6 h from 11 human subjects after each had ingested 5.6 g of PEG showed no interference from endogenous compounds. We find this method to be a simple, labor-saving means of quantifying urinary PEG in the clinical laboratory.
采用“高压”液相色谱法对人尿中不同分子量的聚乙二醇（PEG）进行定量分析。样品需要经过一个简单的制备步骤，即冻干和氯仿萃取。取10微升等分试样，在甲醇和水的等体积混合物中，以等度洗脱方式在苯乙烯二乙烯基苯柱上进行色谱分析，并测量流出物的折射率。结果与标准品浓度在至少10 g/L范围内呈线性变化，在每升总PEG含量为2 g或更高的注入样品中，六种主要组分清晰可辨。低至4 g/L也能精确定量，但为了评估各个组分，我们建议样品最低浓度为7 g/L。添加到尿液对照中的PEG分析回收率为90%。11名人类受试者每人摄入5.6 g PEG后，在6小时内收集的尿液未显示出内源性化合物的干扰。我们发现该方法是临床实验室中定量尿PEG的一种简单、省力的方法。
