Escalante Hermes, Davelois Kelly, Ortiz Pedro, Rodríguez Hans, Díaz Enrique, Jara César
Facultad de Ciencias Biológicas, Universidad Nacional de Trujillo, La Libertad, Perú.
Rev Peru Med Exp Salud Publica. 2011 Jul-Sep;28(3):454-61. doi: 10.1590/s1726-46342011000300008.
To evaluate the performance of the enzyme-linked immunoelectrotransfer blot assay (EITB, Western blot) using excretory/secretory antigens from adult forms of Fasciola hepatica (Fh E/S Ag) for the diagnosis of human fasciolosis.
Antigens were obtained after 18 hours of incubation in culture medium Minimum Essential Eagle, prepared at a protein concentration of 0.15 ug/uL and run against a pool of sera of patients with proven fasciolosis (confirmed by the finding of parasite eggs in the stool microscopy). Antigens of 10, 12, 17, 23, 27, 30, 36, 43, 66 and 136 kDa were detected and used to develop the Western blot technique. The sensitivity was evaluated using sera from 67 fasciolosis patients, and the specificity using sera from 57 patients with other parasitic diseases, and 10 from healthy individuals.
Out of the 67 sera, 64 reacted with the 23 kDa band and 61 with the one of 17 kDa. These two bands were not detected in sera from patients with other parasitic diseases or in those from healthy volunteers and thus could be considered specific and diagnostic.
The sensitivity of the test, using the bands of 17 and 23 kDa, was 95.5% for positive reactions to at least one of these two bands, being its specificity 100% with a positive predictive value of 100% and negative predictive value of 95.71%.
评估使用肝片形吸虫成虫排泄/分泌抗原（Fh E/S Ag）的酶联免疫电转移印迹法（EITB，即蛋白质免疫印迹法）诊断人体肝片吸虫病的性能。
抗原是在最低限度必需培养基中孵育18小时后获得的，制备的蛋白质浓度为0.15微克/微升，并与经证实患有肝片吸虫病患者的血清池进行检测（通过粪便显微镜检查发现寄生虫卵确诊）。检测到分子量为10、12、17、23、27、30、36、43、66和136 kDa的抗原，并用于开发蛋白质免疫印迹技术。使用67例肝片吸虫病患者的血清评估敏感性，使用57例其他寄生虫病患者的血清以及10例健康个体的血清评估特异性。
在67份血清中，64份与23 kDa条带反应，61份与17 kDa条带反应。在其他寄生虫病患者的血清或健康志愿者的血清中未检测到这两条带，因此可认为它们具有特异性和诊断价值。
使用17和23 kDa条带进行检测，该试验的敏感性为95.5%（对这两条带中至少一条呈阳性反应），特异性为100%，阳性预测值为100%，阴性预测值为95.71%。
