患有自发性和实验性诱导白血病的牛体内的牛白血病病毒样病毒特异性序列
BLV-LB virus-specific sequences in cattle with spontaneous and experimentally induced leukemia.
作者信息
Kukaine R A, Nagayeva L I, Dyachenko A G, Chapenko S V, Ilyinskaya T N, Bratsslavskaya O I, Koudeleva G V, Tsibinogin V V, Yakovleva L A, Lapin B A
出版信息
Arch Geschwulstforsch. 1979;49(1):1-5.
Poly(A)-containing RNAs were extracted directly from the culture fluid of BLV-producing continuous and short-term lymphocyte cultures. The abovesaid poly(A)-RNAs were used as a template to synthesize complementary [3H]DNA (BLV cDNA). BLV cDNA possessed a high degree of homology to poly(A)-RNA isolated directly from the blood plasma of a leukemic animal. In contrast of leukemic cattle BLV-related sequences in white blood cells of normal animals were absent. Leukemic cells of cattle with both, spontaneous and experimentally induced leukemia contained bovine leukemia virus sequences in their genomes.
从产生牛白血病病毒（BLV）的连续和短期淋巴细胞培养物的培养液中直接提取含多聚腺苷酸（Poly(A)）的RNA。上述Poly(A) -RNA被用作模板来合成互补的[3H]DNA（BLV cDNA）。BLV cDNA与直接从白血病动物血浆中分离的Poly(A) -RNA具有高度同源性。相比之下，正常动物白细胞中不存在与白血病牛BLV相关的序列。患有自发性和实验性诱导白血病的牛的白血病细胞基因组中都含有牛白血病病毒序列。
Zotero 插件