牛白血病病毒：一种外源性RNA致癌病毒？

Bovine leukemia virus: an exogenous RNA oncogenic virus?

作者信息

Kettmann R, Portetelle D, Mammerickx M, Cleuter Y, Dekegel D, Galoux M, Ghysdael J, Burny A, Chantrenne H

出版信息

Hamatol Bluttransfus. 1976;19:375-89. doi: 10.1007/978-3-642-87524-3_37.

Abstract

Short term cultures of bovine leukemic lymphocytes release virus particles with biochemical properties of RNA oncogenic viruses. These particles, tentatively called Bovine Leukemia Virus (BLV) have a high molecular weight-reverse transcriptase complex and a density averaging 1.155 g/ml in sucrose solutions. Molecular hybridizations between BLV-3H cDNA and several viral RNAs show that BLV is not related to Mason-Pfizer Monkey Virus (MPMV) Simian Sarcoma Associated Virus (SSV-1) Feline Leukemia Virus (FeLV) or Avian Myeloblastosis Virus (AMV). Rauscher Leukemia Virus (RLV) exhibits a slight but reproducible relatednesse to BLV. The high preference of BLV reverse transcriptase for Mg++ as the divalent cation suggests that BLV might be an atypical mammalian leukemogenic type C virus. Hybridization studies using BLV 3H cDNA as a probe suggest that the DNA of bovine leukemic cells contains viral sequences that cannot be detected in normal bovine DNA.

摘要

牛白血病淋巴细胞的短期培养物释放出具有RNA致癌病毒生化特性的病毒颗粒。这些颗粒，暂称为牛白血病病毒（BLV），具有高分子量逆转录酶复合物，在蔗糖溶液中的密度平均为1.155克/毫升。BLV - 3H cDNA与几种病毒RNA之间的分子杂交表明，BLV与梅森 - 菲泽猴病毒（MPMV）、猿猴肉瘤相关病毒（SSV - 1）、猫白血病病毒（FeLV）或禽成髓细胞瘤病毒（AMV）无关。劳舍尔白血病病毒（RLV）与BLV表现出轻微但可重复的相关性。BLV逆转录酶对Mg++作为二价阳离子的高度偏好表明，BLV可能是一种非典型的哺乳动物致白血病C型病毒。使用BLV 3H cDNA作为探针的杂交研究表明，牛白血病细胞的DNA含有在正常牛DNA中无法检测到的病毒序列。