Bio-Oss® 块状物联合 BMP-2 和 VEGF 促进骨缺损和垂直骨增量的再生。

Bio-Oss® blocks combined with BMP-2 and VEGF for the regeneration of bony defects and vertical augmentation.

机构信息

Department of Oral and Maxillofacial Surgery, University of Erlangen-Nuremberg, Erlangen, Germany.

出版信息

Clin Oral Implants Res. 2013 Apr;24(4):450-60. doi: 10.1111/j.1600-0501.2011.02351.x. Epub 2011 Nov 1.

DOI:10.1111/j.1600-0501.2011.02351.x

PMID:22092937

Abstract

OBJECTIVES

The aim of this study was to evaluate the bone formation rate and osseointegration of Bio-Oss(®) blocks combined with rhBMP-2 and rhVEGF in bony defects and after vertical augmentation.

MATERIAL AND METHODS

Bio-Oss(®) blocks plus rhBMP-2 (BMP), Bio-Oss(®) blocks plus rhVEGF (VEGF), or Bio-Oss(®) blocks plus rhBMP-2 and rhVEGF (BMPVEGF) were inserted in "critical size defects" (CSD) in the calvariae of adult pigs. Control defects were filled with collagen carrier (Lyostypt(®) ) plus growth factors and untreated Bio-Oss(®) blocks (CO). In a second group, Bio-Oss(®) blocks plus growth factors and untreated Bio-Oss(®) blocks were used for vertical augmentation of the calvariae. In the first group, the investigation time was 30 days, in the second group it was 30 and 60 days. The bone samples were investigated histomorphometrically, and the newly formed bone (BV/TV) was judged by microradiographic investigation.

RESULTS

In the CSD model, the newly formed bone in the region of interest was not significantly different within the groups. In the second setting, the inserted bone blocks exhibited sufficient volume stability with increasing bone formation up to 9.33% ± 3.92% for BMP, 10.42% ± 1.81% for BMP/VEGF, 11.01% ± 4.78% for VEGF, and 10.02% ± 5.43% for the control group after 60 days.

CONCLUSION

In the chosen setting and time frame, de novo bone formation did not increase with the additional use of growth factors.

摘要

目的

本研究旨在评估 Bio-Oss ® （骨基质明胶）块联合 rhBMP-2 和 rhVEGF 在骨缺损和垂直骨增量后的成骨率和骨整合情况。

材料和方法

Bio-Oss ® （骨基质明胶）块联合 rhBMP-2（BMP）、Bio-Oss ® （骨基质明胶）块联合 rhVEGF（VEGF）或 Bio-Oss ® （骨基质明胶）块联合 rhBMP-2 和 rhVEGF（BMPVEGF）被植入成年猪颅骨的“临界尺寸缺损”（CSD）中。对照缺损用胶原载体（Lyostypt ® ）加生长因子和未处理的 Bio-Oss ® （骨基质明胶）块（CO）填充。在第二组中，Bio-Oss ® （骨基质明胶）块联合生长因子和未处理的 Bio-Oss ® （骨基质明胶）块被用于颅骨的垂直骨增量。在第一组中，研究时间为 30 天，在第二组中为 30 天和 60 天。通过组织形态计量学研究对骨样本进行了评估，并通过微射线照相研究判断新形成的骨（BV/TV）。

结果

在 CSD 模型中，感兴趣区域内各组之间新形成的骨没有显著差异。在第二组中，插入的骨块表现出足够的体积稳定性，随着骨形成的增加，BMP 组为 9.33%±3.92%，BMP/VEGF 组为 10.42%±1.81%，VEGF 组为 11.01%±4.78%，对照组为 10.02%±5.43%，至 60 天时。