Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo (USP), Av. do Café s/n, Monte Alegre, Ribeirão Preto, SP, 14040-904, Brazil.
Clin Oral Investig. 2018 Jul;22(6):2335-2343. doi: 10.1007/s00784-018-2334-0. Epub 2018 Jan 18.
This investigation aimed to characterize in a 6-month follow-up the microbial profile of implants restored with either titanium or zirconia abutments at the genus or higher taxonomic levels.
Twenty healthy individuals indicative for implant-retained single restorations were investigated. Half of participants were restored with titanium and half with zirconia abutments. Biofilm was collected from the implant-related sites after 1, 3, and 6 months of loading. The 16S rDNA genes were amplified and sequenced with Roche/454 platform.
A total of 596 species were identified in 360 samples and grouped in 18 phyla and 104 genera. Titanium- or zirconia-related sites as well as teeth showed similar total numbers of operational taxonomic units (OTUs) colonizing surfaces over time. Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes, and Actinobacteria were the most prevalent phyla with significant differences between different surfaces and time point. Unclassified genera were found in lower levels (1.71% up to 9.57%) on titanium and zirconia samples when compared with teeth, with no significant differences.
Titanium- and zirconia-related surfaces are promptly colonized by a bacterial community similar to those found in the remaining adjacent teeth. Results suggest a selective adhesion of different bacterial genotypes for either titanium or zirconia surfaces. Data also indicate a significant interaction between the relative effects taxa, time point, and sampling site.
The present study disclosed a wider spectrum of microorganisms colonizing either titanium- or zirconia-related microbiomes in very early stage of implant colonization, revealing differences and suggesting a probably specific mechanism for selective bacterial adhesion.
本研究旨在 6 个月的随访中，以属或更高分类水平来描述用钛或氧化锆基台修复的种植体的微生物特征。
本研究共纳入 20 名健康患者，这些患者均有单个种植体修复指征。其中，一半患者采用钛基台修复，另一半患者采用氧化锆基台修复。在负荷 1、3 和 6 个月后，从种植体相关部位采集生物膜。采用罗氏/454 平台扩增和测序 16S rDNA 基因。
在 360 个样本中，共鉴定出 596 个物种，分为 18 门和 104 属。随着时间的推移，钛或氧化锆相关部位和牙齿表面的总定植菌数量相似。厚壁菌门、变形菌门、梭杆菌门、拟杆菌门和放线菌门是最常见的门，不同表面和时间点之间存在显著差异。与牙齿相比，钛和氧化锆样本上未分类属的数量较低（1.71%至 9.57%），但无统计学差异。
钛和氧化锆相关表面很快被类似于相邻牙齿上发现的细菌群落定植。结果表明，不同细菌基因型对钛或氧化锆表面具有选择性粘附。数据还表明，在相对效应分类群、时间点和采样部位之间存在显著的相互作用。
本研究在种植体早期定植阶段，揭示了更多定植于钛或氧化锆相关微生物组的微生物，显示了差异，并提示了一种可能的特定细菌选择性粘附机制。
