Neuman R G, Bonomini L V, Braunstein S N
Exocell, Inc., Philadelphia, PA 19104.
Diabetes Care. 1990 Oct;13(10):1069-73. doi: 10.2337/diacare.13.10.1069.
A novel enzyme-linked immunosorbent assay (ELISA [Dialbumin]) for rapid office measurement of microalbuminuria was evaluated and its performance compared with that of a commercially available radioimmunoassay (double-antibody albumin). Urine samples containing between 0.75 and 1800 micrograms/ml of albumin were obtained from 31 diabetic patients and assayed by both methods. A comparison of the paired values obtained from the two methods gave a correlation coefficient of greater than 0.99. The Dialbumin assay, which used detachable eight-well strips (1 strip/sample), 10-min incubation, tap water wash, and a 2-min color development step, was read on both an ELISA reader and a hand-held analytical device (Acc-U-Dial) designed specifically for this test. The findings of this study indicate that the Dialbumin assay, used in conjunction with the Acc-U-Dial device, affords a rapid, convenient, and sensitive method for quantitative determination of a broad range (0.3-1280 micrograms/ml) of urinary albumin levels in the office setting.
一种用于在门诊快速检测微量白蛋白尿的新型酶联免疫吸附测定法（ELISA[双白蛋白法]）进行了评估，并将其性能与市售放射免疫测定法（双抗体白蛋白法）进行了比较。从31名糖尿病患者中获取了白蛋白含量在0.75至1800微克/毫升之间的尿液样本，并采用两种方法进行检测。对两种方法获得的配对值进行比较，相关系数大于0.99。双白蛋白测定法使用可拆卸的八孔条带（每条带用于一个样本），孵育10分钟，用自来水冲洗，并进行2分钟的显色步骤，可在酶联免疫吸附测定仪和专门为此测试设计的手持式分析设备（Acc-U-Dial）上读取结果。本研究结果表明，双白蛋白测定法与Acc-U-Dial设备联合使用，为在门诊环境中定量测定广泛范围（0.3 - 1280微克/毫升）的尿白蛋白水平提供了一种快速、便捷且灵敏的方法。
