Laboratory of Fermentation Technology, Department of Microbiology, Kurukshetra University, Kurukshetra, Haryana, 136119, India.
Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, 136119, India.
Arch Microbiol. 2023 Mar 27;205(4):146. doi: 10.1007/s00203-023-03468-1.
Rice straw is a suitable alternative to a cheaper carbohydrate source for the production of ethanol. For pretreatment efficiency, different sodium hydroxide concentrations (0.5-2.5% w/v) were tested. When compared to other concentrations, rice straw processed with 2% NaOH (w/v) yielded more sugar (8.17 ± 0.01 mg/ml). An alkali treatment induces effective delignification and swelling of biomass. The pretreatment of rice straw with 2% sodium hydroxide (w/v) is able to achieve 55.34% delignification with 53.30% cellulose enrichment. The current study shows the effectiveness of crude cellulolytic preparation from Aspergillus niger resulting in 80.51 ± 0.4% cellulose hydrolysis. Rice straw hydrolysate was fermented using ethanologenic Saccharomyces cerevisiae (yeast) and Zymomonas mobilis (bacteria). Overall, superior efficiency of sugar conversion to ethanol 70.34 ± 0.3% was obtained with the yeast compared to bacterial strain 39.18 ± 0.5%. The current study showed that pretreatment with sodium hydroxide is an effective method for producing ethanol from rice straw and yeast strain S. cerevisiae having greater fermentative potential for bioethanol production than bacterial strain Z. mobilis.
稻草是一种比更廉价的碳水化合物原料更适合生产乙醇的替代品。为了提高预处理效率，我们测试了不同浓度的氢氧化钠（0.5-2.5% w/v）。与其他浓度相比，用 2%氢氧化钠（w/v）处理的稻草产生了更多的糖（8.17 ± 0.01 mg/ml）。碱处理能有效地脱木质素并使生物质膨胀。用 2%氢氧化钠（w/v）预处理稻草可达到 55.34%的脱木质素和 53.30%的纤维素富集。本研究表明，黑曲霉粗纤维素酶制剂能有效水解纤维素，水解率达到 80.51 ± 0.4%。采用产乙醇酵母酿酒酵母（yeast）和运动发酵单胞菌（bacteria）对稻草水解液进行发酵。总的来说，与细菌菌株 39.18 ± 0.5%相比，酵母的糖到乙醇转化率为 70.34 ± 0.3%，效率更高。本研究表明，氢氧化钠预处理是从稻草生产乙醇的有效方法，而酿酒酵母菌株 S. cerevisiae 比细菌菌株 Z. mobilis 具有更高的发酵生产生物乙醇的潜力。
