硫酸铜和维生素 C 对全白茶和亚组分白茶的抗菌活性增强作用及其对金黄色葡萄球菌的作用机制。

Enhancement of antimicrobial activities of whole and sub-fractionated white tea by addition of copper (II) sulphate and vitamin C against Staphylococcus aureus; a mechanistic approach.

机构信息

School of Life Sciences, Kingston University, Kingston, London, UK.

出版信息

BMC Complement Altern Med. 2011 Nov 17;11:115. doi: 10.1186/1472-6882-11-115.

DOI:10.1186/1472-6882-11-115

PMID:22093997

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3239241/

Abstract

BACKGROUND

Enhancement of antimicrobial plant products e.g. pomegranate extract by copper (II) sulphate is known. Such combinations have applications in various settings, including the identification of novel compositions to study, treat and control infection.

METHODS

A combination of white tea (WT) (made allowing 10 minutes infusion time at 100°C) was combined with 4.8 mM copper (II) sulphate and tested for antimicrobial effect on the viability of Staphylococcus aureus NCTC 06571. Comparisons were made with green (GT) and black (BT) teas. A WT sub-fraction (WTF < 1000 Da) was tested with copper (II) sulphate and 4.8 mM vitamin C. pH measurements of samples were taken for controls and to observe any changes due to tea/agent interaction. Catalase was used to investigate hydrogen peroxide release. UV-vis. was used to compare WT and WTF.

RESULTS

A 30 minute incubation at room temperature of copper (II) sulphate alone and combined with WT reduced the viability of S. aureus NCTC 06571 by c.a 1 log10 cfu mL-1. GT and BT with copper (II) sulphate negated activity to buffer values. Combined with copper (II) sulphate, vitamin C, WTF and, vitamin C plus WTF all reduced the viability of S. aureus NCTC 06571 by c.a. 3.5 log10 cfu mL-1. Independent experiments showed the results were not due to pH effects. Adding WT or WTF to copper (II) sulphate resulted in increased acidity. Copper (II) sulphate alone and combined with WT required c.a 300 μg mL-1 (final concentration) catalase to restore S. aureus viability, WTF with copper (II) sulphate and added vitamin C required c.a 600 μg mL-1. WT and WTF UV-visible spectra were similar.

CONCLUSIONS

WT showed no efficacy in the combinations tested. WTF was enhanced with copper (II) sulphate and further with vitamin C. WT and WTF increased acidity of copper (II) sulphate possibly via the formation of chemical complexes. The difference in WT/WTF absorbance possibly represented substances less concentrated or absent in WTF. Investigations to establish which WTF component/s and in what proportions additives are most effective against target organisms are warranted.

摘要

背景

人们已知，通过添加硫酸铜可以增强抗菌植物提取物（如石榴提取物）的功效。这些组合在各种环境中都有应用，包括鉴定新的组合物以研究、治疗和控制感染。

方法

将白茶（WT）（允许在 100°C 下浸泡 10 分钟）与 4.8mM 硫酸铜混合，并测试其对金黄色葡萄球菌 NCTC 06571 存活能力的抗菌效果。将其与绿茶（GT）和黑茶（BT）进行了比较。测试了 WT 的一个亚组分（WTF<1000Da）与硫酸铜和 4.8mM 维生素 C 的组合。为了控制并观察由于茶/试剂相互作用引起的任何变化，对样品进行了 pH 测量。用过氧化氢酶来研究过氧化氢的释放。使用紫外-可见分光光度计比较 WT 和 WTF。

结果

在室温下单独使用硫酸铜以及与 WT 一起孵育 30 分钟，使金黄色葡萄球菌 NCTC 06571 的存活能力降低了约 1 个对数 10 cfu mL-1。与硫酸铜结合的 GT 和 BT 则使活性降至缓冲值。与硫酸铜结合，维生素 C、WTF 以及维生素 C 加 WTF 均使金黄色葡萄球菌 NCTC 06571 的存活能力降低了约 3.5 个对数 10 cfu mL-1。独立实验表明，结果不是由于 pH 值的影响。向硫酸铜中添加 WT 或 WTF 会导致酸度增加。硫酸铜单独使用以及与 WT 结合使用需要约 300μg mL-1（最终浓度）的过氧化氢酶才能恢复金黄色葡萄球菌的活力，而与硫酸铜结合使用的 WTF 和添加的维生素 C 则需要约 600μg mL-1。WT 和 WTF 的紫外-可见光谱相似。

结论

WT 在测试的组合中没有效果。WTF 与硫酸铜结合后，与维生素 C 结合后效果进一步增强。WT 和 WTF 增加了硫酸铜的酸度，可能是通过形成化学络合物。WT/WTF 吸光度的差异可能代表 WTF 中浓度较低或不存在的物质。有必要进行研究以确定哪种 WTF 成分以及以何种比例添加物对目标生物最有效。