Gould Simon W J, Fielder Mark D, Kelly Alison F, Naughton Declan P
School of Life Sciences, Kingston University, Kingston upon Thames, London KT1 2EE, UK.
BMC Complement Altern Med. 2009 Jul 27;9:23. doi: 10.1186/1472-6882-9-23.
Recently, natural products have been evaluated as sources of antimicrobial agents with efficacies against a variety of micro-organisms.
This report describes the antimicrobial activities of pomegranate rind extract (PRE) singularly and in combination with cupric sulphate against methicillin-sensitive and -resistant Staphylococcus aureus (MSSA, MRSA respectively), and Panton-Valentine Leukocidin positive community acquired MSSA (PVL positive CA-MSSA).
PRE alone showed limited efficacy against MRSA and MSSA strains. Exposure to copper (II) ions alone for 2 hours resulted in moderate activity of between 102 to 103 log10 cfu mL-1 reduction in growth. This was enhanced by the addition of PRE to 104 log10 cfu mL-1 reduction in growth being observed in 80% of the isolates. However, the PVL positive CA-MSSA strains were more sensitive to copper (II) ions which exhibited moderate activities of between 103 log10 cfu mL-1 reduction in growth for 60% of the isolates.
PRE, in combination with Cu(II) ions, was seen to exhibit moderate antimicrobial effects against clinical isolates of MSSA, MRSA and PVL positive CA-MSSA isolates. The results of this study indicate that further investigation into the active ingredients of natural products, their mode of action and potential synergism with other antimicrobial agents is warranted. This is the first report of the efficacy of pomegranate against clinical PVL positive CA-MSSA isolates.
最近，天然产物已被评估为具有抗多种微生物功效的抗菌剂来源。
本报告描述了石榴皮提取物（PRE）单独以及与硫酸铜联合对甲氧西林敏感和耐药金黄色葡萄球菌（分别为MSSA、MRSA）以及社区获得性MSSA中Panton-Valentine杀白细胞素阳性菌株（PVL阳性CA-MSSA）的抗菌活性。
单独使用PRE对MRSA和MSSA菌株显示出有限的功效。单独暴露于铜（II）离子2小时导致生长减少10²至10³ log₁₀ cfu mL⁻¹的中度活性。通过添加PRE，在80%的分离株中观察到生长减少至10⁴ log₁₀ cfu mL⁻¹，活性增强。然而，PVL阳性CA-MSSA菌株对铜（II）离子更敏感，60%的分离株生长减少10³ log₁₀ cfu mL⁻¹，表现出中度活性。
PRE与Cu（II）离子联合对MSSA、MRSA和PVL阳性CA-MSSA临床分离株表现出中度抗菌作用。本研究结果表明有必要进一步研究天然产物的活性成分、其作用方式以及与其他抗菌剂的潜在协同作用。这是石榴对临床PVL阳性CA-MSSA分离株功效的首次报告。
