牙髓病学中的光活化消毒（PAD）：一项体外微生物学评估。
Photoactivated disinfection (PAD) in endodontics: an in vitro microbiological evaluation.
作者信息
Poggio Claudio, Arciola Carla Renata, Dagna Alberto, Florindi Filippo, Chiesa Marco, Saino Enrica, Imbriani Marcello, Visai Livia
机构信息
Department of Endodontics, University of Pavia, Pavia, Italy.
出版信息
Int J Artif Organs. 2011 Sep;34(9):889-97. doi: 10.5301/ijao.5000056.
PURPOSE
The objective of the present study was the in vitro evaluation by MTT test of the antimicrobial effect of photoactivated disinfection (PAD) and, comparatively, of a conventional 5.25% NaOCl irrigating solution.
METHODS
Enterococcus faecalis, Streptococcus mutans and Streptococcus sanguis strains were selected for the test. Freshly extracted single-rooted human teeth were endodontically treated, inoculated with bacterial strains and then divided into different groups, each of them treated with PAD, with PAD plus 0.5% NaOCl solution, with TBO, with PAD for longer time and with 5% NaOCl solution (positive control).
RESULTS
The results were significantly different among the various groups, and for Enterococcus faecalis, Streptococcus mutans and Streptococcus sanguis. PAD applied for a longer time (in respect to manufacturer's instructions) or PAD associated to 5% NaOCl showed the significantly higher antibacterial effects.
目的
本研究的目的是通过MTT试验对光活化消毒（PAD）的抗菌效果以及传统的5.25%次氯酸钠冲洗液进行体外评估，并进行比较。
方法
选择粪肠球菌、变形链球菌和血链球菌菌株进行试验。将新鲜拔除的单根人牙进行根管治疗，接种细菌菌株，然后分为不同组，每组分别用PAD、PAD加0.5%次氯酸钠溶液、亚甲蓝、延长时间的PAD以及5%次氯酸钠溶液（阳性对照）进行处理。
结果
不同组之间以及粪肠球菌、变形链球菌和血链球菌之间的结果存在显著差异。延长时间应用的PAD（相对于制造商说明）或与5%次氯酸钠联合应用的PAD显示出显著更高的抗菌效果。
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