Poggio Claudio, Arciola Carla Renata, Dagna Alberto, Florindi Filippo, Chiesa Marco, Saino Enrica, Imbriani Marcello, Visai Livia
Department of Endodontics, University of Pavia, Pavia, Italy.
Int J Artif Organs. 2011 Sep;34(9):889-97. doi: 10.5301/ijao.5000056.
The objective of the present study was the in vitro evaluation by MTT test of the antimicrobial effect of photoactivated disinfection (PAD) and, comparatively, of a conventional 5.25% NaOCl irrigating solution.
Enterococcus faecalis, Streptococcus mutans and Streptococcus sanguis strains were selected for the test. Freshly extracted single-rooted human teeth were endodontically treated, inoculated with bacterial strains and then divided into different groups, each of them treated with PAD, with PAD plus 0.5% NaOCl solution, with TBO, with PAD for longer time and with 5% NaOCl solution (positive control).
The results were significantly different among the various groups, and for Enterococcus faecalis, Streptococcus mutans and Streptococcus sanguis. PAD applied for a longer time (in respect to manufacturer's instructions) or PAD associated to 5% NaOCl showed the significantly higher antibacterial effects.
本研究的目的是通过MTT试验对光活化消毒（PAD）的抗菌效果以及传统的5.25%次氯酸钠冲洗液进行体外评估，并进行比较。
选择粪肠球菌、变形链球菌和血链球菌菌株进行试验。将新鲜拔除的单根人牙进行根管治疗，接种细菌菌株，然后分为不同组，每组分别用PAD、PAD加0.5%次氯酸钠溶液、亚甲蓝、延长时间的PAD以及5%次氯酸钠溶液（阳性对照）进行处理。
不同组之间以及粪肠球菌、变形链球菌和血链球菌之间的结果存在显著差异。延长时间应用的PAD（相对于制造商说明）或与5%次氯酸钠联合应用的PAD显示出显著更高的抗菌效果。
