Vaid Deepa, Shah Nimisha, Kothari Dwij, Bilgi Priyanka
Department of Conservative Dentistry and Endodontics, K. M. Shah Dental College, Sumandeep Vidyapeeth, Vadodara, Gujarat, India.
Department of Prosthodontics, Darshan Dental College, Udaipur, Rajasthan, India.
J Conserv Dent. 2017 Jan-Feb;20(1):41-45. doi: 10.4103/0972-0707.209072.
Evaluation of the additive effect of photodynamic therapy (PDT) on the antibacterial activity of 2.5% sodium hypochlorite (NaOCl) and QMix against 6-week biofilms contaminated root canals.
To establish the most suitable irrigant for eradication of 6-week biofilms.
study.
A 6-week (ATCC 29212) biofilm was formed in 190 extracted teeth that were subsequently subjected to irrigation protocols as follows. Group A: normal saline, Group A: 2.5% NaOCl, Group A: QMix, Group B: normal saline and photoactivated disinfection (PAD), Group B: 2.5% NaOCl and PAD, Group B: QMix and PAD, Group C: no irrigation. For PAD, irradiation was done three times for 5 s each with 10 s interval on continuous mode with a 980 nm diode laser. Samples from the root canals were collected and plated onto brain heart infusion agar plates to determine the colony-forming unit/ml.
One-way ANOVA, post hoc Tukey's honest significant difference test.
Maximum percentage of disinfection (99%) was seen in Group B (NaOCl with PDT), which was similar to Groups A (97.6%) and B (98.8%) ( < 0.0001).
NaOCl with PDT gave maximum disinfection.
评估光动力疗法（PDT）对2.5%次氯酸钠（NaOCl）和QMix针对六周龄根管污染生物膜抗菌活性的增效作用。
确定根除六周龄生物膜的最合适冲洗液。
研究。
在190颗拔除的牙齿中形成六周龄（ATCC 29212）生物膜，随后对其进行如下冲洗方案。A组：生理盐水，A组：2.5% NaOCl，A组：QMix，B组：生理盐水和光活化消毒（PAD），B组：2.5% NaOCl和PAD，B组：QMix和PAD，C组：不冲洗。对于PAD，使用980 nm二极管激光器以连续模式每次照射5秒，间隔10秒，共照射三次。从根管中收集样本并接种到脑心浸液琼脂平板上以确定菌落形成单位/毫升。
单因素方差分析，事后Tukey诚实显著差异检验。
B组（NaOCl联合PDT）的消毒百分比最高（99%），与A组（97.6%）和B组（98.8%）相似（<0.0001）。
NaOCl联合PDT消毒效果最佳。
