Dagna Alberto, Arciola Carla Renata, Florindi Filippo, Scribante Andrea, Saino Enrica, Visai Livia, Poggio Claudio
Department of Endodontics, Policlinico San Matteo, University of Pavia, Pavia, Italy.
Int J Artif Organs. 2011 Sep;34(9):914-9. doi: 10.5301/ijao.5000049.
The objective of this study was to compare in vitro, by MTT assay, the antimicrobial efficacy of Niclor 5 (5% NaOCl solution), Cloreximid (0.2% chlorhexidine and 0.2% cetrimide solution), 3% hydrogen peroxide and 17% EDTA against two microorganisms associated with primary endodontic infections.
Enterococcus faecalis and Streptococcus mutans strains were selected for this test. Freshly extracted single-rooted human teeth were endodontically treated, inoculated with bacterial strains and then divided into different groups, each of them rinsed with Niclor 5 (5% NaOCl solution), Cloreximid (0.2% chlorhexidine and 0.2% cetrimide solution), 3% hydrogen peroxide,17% EDTA and with 5% NaOCl solution (positive control).
Even though all the tested irrigating solutions demonstrated antibacterial effects against E. faecalis and S. mutans, the results were significantly different between the various groups. The greatest antimicrobial effects were observed in groups treated with 5% NaOCl and 17% EDTA. Interestingly, the effectiveness of EDTA could be ascribed to its capability of detaching biofilm from canal walls.
本研究的目的是通过MTT法在体外比较5%次氯酸钠溶液（尼氯5）、氯己定洗必泰（0.2%氯己定和0.2%西曲溴铵溶液）、3%过氧化氢和17%乙二胺四乙酸（EDTA）对两种与原发性牙髓感染相关的微生物的抗菌效果。
选择粪肠球菌和变形链球菌菌株进行此项试验。将新鲜拔除的单根人牙进行牙髓治疗，接种细菌菌株，然后分成不同组，每组分别用尼氯5（5%次氯酸钠溶液）、氯己定洗必泰（0.2%氯己定和0.2%西曲溴铵溶液）、3%过氧化氢、17% EDTA以及5%次氯酸钠溶液（阳性对照）冲洗。
尽管所有测试的冲洗液对粪肠球菌和变形链球菌均显示出抗菌作用，但不同组之间的结果存在显著差异。在用5%次氯酸钠和17% EDTA处理的组中观察到最大的抗菌效果。有趣的是，EDTA的有效性可归因于其从根管壁分离生物膜的能力。
