Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, Japan.
Cell Tissue Res. 2012 Mar;347(3):775-82. doi: 10.1007/s00441-011-1267-6. Epub 2011 Nov 18.
Regenerative therapies, including cell injection and bioengineered tissue transplantation, have the potential to treat severe heart failure. Direct implantation of isolated skeletal myoblasts and bone-marrow-derived cells has already been clinically performed and research on fabricating three-dimensional (3-D) cardiac grafts using tissue engineering technologies has also now been initiated. In contrast to conventional scaffold-based methods, we have proposed cell sheet-based tissue engineering, which involves stacking confluently cultured cell sheets to construct 3-D cell-dense tissues. Upon layering, individual cardiac cell sheets integrate to form a single, continuous, cell-dense tissue that resembles native cardiac tissue. The transplantation of layered cardiac cell sheets is able to repair damaged hearts. As the next step, we have attempted to promote neovascularization within bioengineered myocardial tissues to overcome the longstanding limitations of engineered tissue thickness. Finally, as a possible advanced therapy, we are now trying to fabricate functional myocardial tubes that may have a potential for circulatory support. Cell sheet-based tissue engineering technologies therefore show an enormous promise as a novel approach in the field of myocardial tissue engineering.
再生疗法，包括细胞注射和生物工程组织移植，具有治疗严重心力衰竭的潜力。已经在临床上进行了分离的骨骼肌成肌细胞和骨髓来源细胞的直接植入，并且现在已经开始使用组织工程技术制造三维（3-D）心脏移植物的研究。与传统的基于支架的方法不同，我们提出了基于细胞片的组织工程，其涉及堆叠汇合培养的细胞片以构建 3-D 细胞密集组织。在分层时，单个心脏细胞片整合形成单个连续的细胞密集组织，类似于天然心脏组织。分层心脏细胞片的移植能够修复受损的心脏。作为下一步，我们试图在生物工程心肌组织内促进新血管生成，以克服工程组织厚度的长期限制。最后，作为一种可能的先进疗法，我们现在正试图制造功能性心肌管，这些心肌管可能具有循环支持的潜力。因此，基于细胞片的组织工程技术作为心肌组织工程领域的一种新方法具有巨大的潜力。
