Centre Scientifique et Technique du Bâtiment, 84 avenue Jean Jaurès, Champs-sur-Marne, 77447 Marne-la-Vallée cedex 02, France.
Water Sci Technol. 2011;64(3):708-14. doi: 10.2166/wst.2011.696.
Legionella bacteria encounter optimum growing conditions in hot water systems and cooling towers. A pilot-scale 1 unit was built in order to study the biofilm disinfection. It consisted of two identical loops, one used as a control and the other as a 'Test Loop'. A combination of a bio-detergent and a biocide (hydrogen peroxide + peracetic acid) was applied in the Test Loop three times under the same conditions at 100 and 1,000 mg/L with a contact time of 24 and 3-6 hours, respectively. Each treatment test was preceded by a three week period of biofilm re-colonization. Initial concentrations of culturable Legionella into biofilm were close to 10(3) CFU/cm2. Results showed that culturable Legionella spp. in biofilm were no longer detectable three days following each treatment. evertheless, initial Legionella spp. concentrations were recovered 7 days after the treatments (in two cases). Before the tests, Legionella spp. and L. pneumophila PCR counts were both about 10(4) GU/cm2 in biofilm and they both decreased by 1 to 2 log units 72 hours after each treatment. The three tests had a good but transient efficiency on Legionella disinfection in biofilm.
军团菌在热水系统和冷却塔中遇到最佳生长条件。为了研究生物膜的消毒，建立了一个中试规模的 1 个单元。它由两个相同的回路组成，一个用作对照，另一个用作“测试回路”。在相同条件下，以 100 和 1000 mg/L 的浓度，接触时间分别为 24 小时和 3-6 小时，三次向测试回路中添加了生物清洁剂和杀生剂（过氧化氢+过氧乙酸）的混合物。每次处理试验前，都有一个为期三周的生物膜再定植期。初始可培养军团菌的浓度接近 10(3)CFU/cm2。结果表明，每次处理后三天，生物膜中的可培养军团菌均无法检测到。然而，在处理后 7 天（两种情况下），初始军团菌的浓度又恢复了。在试验前，生物膜中的军团菌和嗜肺军团菌的 PCR 计数均约为 10(4)GU/cm2，每次处理后 72 小时，数量均减少了 1 到 2 个对数单位。三次试验对生物膜中军团菌的消毒效果良好，但只是暂时的。
