Fukamizu H, Grinnell F
Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical School, Dallas 75235.
Exp Cell Res. 1990 Oct;190(2):276-82. doi: 10.1016/0014-4827(90)90197-i.
The goal of our research is to understand reciprocal relationships between cell function and tissue organization. We studied the regulation of fibroblast activity in an in vitro culture model that recapitulates in continuous fashion the cycle of events occurring during connective tissue repair. We present evidence that concomitant with spatial reorganization of the extracellular matrix, there was a dramatic decline in extracellular matrix synthesis and cell proliferation. Therefore, spatial reorganization was a crucial turning point for fibroblast activity. Factors that regulated the timing of spatial reorganization included serum, transforming growth factor beta, and fibronectin. By accelerating spatial reorganization of the cultures, transforming growth factor beta led to a relative decrease in cell proliferation and extracellular matrix synthesis. By retarding spatial reorganization of the cultures, fibronectin led to a relative increase in cell proliferation and extracellular matrix synthesis. The results indicate that spatial information in the three-dimensional cell-matrix interaction permits higher order, tissue-level regulation of fibroblast function.
我们研究的目标是了解细胞功能与组织结构之间的相互关系。我们在一个体外培养模型中研究了成纤维细胞活性的调节，该模型以连续的方式重现了结缔组织修复过程中发生的一系列事件。我们提供的证据表明，伴随着细胞外基质的空间重组，细胞外基质合成和细胞增殖显著下降。因此，空间重组是成纤维细胞活性的关键转折点。调节空间重组时间的因素包括血清、转化生长因子β和纤连蛋白。通过加速培养物的空间重组，转化生长因子β导致细胞增殖和细胞外基质合成相对减少。通过延缓培养物的空间重组，纤连蛋白导致细胞增殖和细胞外基质合成相对增加。结果表明，三维细胞-基质相互作用中的空间信息允许对成纤维细胞功能进行更高层次的组织水平调节。
