血小板裂解液：动物细胞培养中替代胎牛血清的物质？
Platelet lysate: a replacement for fetal bovine serum in animal cell culture?
机构信息
Department of Chemistry and Biomedical Sciences, University of Kalmar, SE-391 82, Kalmar, Sweden.
出版信息
Cytotechnology. 2003 Jul;42(2):67-74. doi: 10.1023/B:CYTO.0000009820.72920.cf.
A new cell culture supplement, platelet lysate, was evaluated with reference to fetal bovine serum (FBS), an established industrial medium for animal cell culture. Chemical and bacteriological profiles were conducted including the presence of platelet-derived growth factor (PDGF). PDGF was detected in the platelet lysate but it was not present in FBS. The platelet lysate medium demonstrated lack of microorganisms, mycoplasma and endotoxins. The platelet lysate was investigated in culture studies (cell growth, viability and product formation) towards a number of target cells including myelomas, hybridomas, hepatocytes, fibroblasts and epithelial cells. In general the platelet lysate medium supported cell growth and maintained viabilities comparable or superior to fetal bovine serum. Productivity studies of antibodies (hybridomas) and transferrin (hepatocytes) showed similar or enhanced production in platelet-derived medium in comparison with FBS.
一种新的细胞培养补充剂，血小板裂解液，被评估与胎牛血清（FBS）参考，一个既定的动物细胞培养的工业培养基。进行了化学和细菌学分析，包括血小板衍生生长因子（PDGF）的存在。血小板裂解液中检测到 PDGF，但 FBS 中不存在。血小板裂解液培养基中缺乏微生物、支原体和内毒素。对包括骨髓瘤、杂交瘤、肝细胞、成纤维细胞和上皮细胞在内的多种靶细胞进行了培养研究（细胞生长、活力和产物形成），以研究血小板裂解液。总的来说，血小板裂解液培养基支持细胞生长，维持的活力与胎牛血清相当或更高。抗体（杂交瘤）和转铁蛋白（肝细胞）的生产力研究表明，与 FBS 相比，血小板衍生培养基中的产量相似或增强。
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