Stephens P, al-Khateeb T, Davies K J, Shepherd J P, Thomas D W
Department of Oral Surgery, Medicine and Pathology, University of Wales College of Medicine, Cardiff, UK.
Int J Oral Maxillofac Surg. 1996 Apr;25(2):161-4. doi: 10.1016/s0901-5027(96)80065-8.
This study investigated the effect of clinical concentrations of alcohol on fibroblast function (proliferation and ECM synthesis) in vitro. Basal and TGF-beta-induced collagen synthesis was assayed in confluent cultures in serum-free medium at 48 h with a commercial collagen assay system. At concentrations of alcohol > 5%, fibroblast proliferation was significantly inhibited. Although noninhibitory, subclinical concentrations of alcohol failed to inhibit basal collagen synthesis (P > 0.1), they significantly decreased TGF-beta-induced collagen synthesis (P < 0.03). These data support the notion that the local, as well as the systemic, effects of alcohol are important in mediating delayed healing in alcoholic patients.
本研究调查了临床浓度酒精对体外成纤维细胞功能（增殖和细胞外基质合成）的影响。使用商业胶原蛋白检测系统，在无血清培养基中汇合培养48小时后，测定基础和转化生长因子-β（TGF-β）诱导的胶原蛋白合成。当酒精浓度>5%时，成纤维细胞增殖受到显著抑制。虽然亚临床浓度的酒精无抑制作用，但未能抑制基础胶原蛋白合成（P>0.1），却显著降低了TGF-β诱导的胶原蛋白合成（P<0.03）。这些数据支持了这样一种观点，即酒精的局部和全身作用在介导酒精性患者愈合延迟方面都很重要。
