Department of Surgical Science, Karolinska Institutet, S-171 77, Stockholm, Sweden.
Cytotechnology. 2005 Jun;48(1-3):89-95. doi: 10.1007/s10616-005-4074-7.
Cell- and tissue culture methodology form an important base for biological, biochemical and biomedical research. Most cell culture techniques require the use of animal sera for the successful propagation of cells. However, the varying composition between batches has fuelled the need for alternatives. In the case of serum derived from animal foetuses, ethical concerns have also been raised. Here we compare the use of a platelet derived lysate (Plysate), which is currently under development as a serum substitute, in the culturing of primary human muscle cells to foetal bovine serum (FBS). In cells cultured with Plysate, differentiation into myotubes, glucose-uptake, phosphatidylinositol 3-kinase (PI3K) activity and expression and phosphorylation of ERK1/2 MAPK and PKB/Akt was impaired. Thus for primary human skeletal muscle Plysate is a sub-optimal substitute for FBS.
细胞和组织培养方法是生物、生化和生物医学研究的重要基础。大多数细胞培养技术都需要使用动物血清来成功繁殖细胞。然而，批次之间的成分变化促使人们需要替代品。在动物胎牛血清的情况下，也引起了伦理问题的关注。在这里，我们比较了血小板衍生裂解物（Plysate）的使用，它目前正在开发中作为血清替代品，用于培养原代人肌肉细胞与胎牛血清（FBS）。在用 Plysate 培养的细胞中，向肌管分化、葡萄糖摄取、磷脂酰肌醇 3-激酶（PI3K）活性以及 ERK1/2 MAPK 和 PKB/Akt 的表达和磷酸化受到损害。因此，对于原代人骨骼肌来说，Plysate 是 FBS 的次优替代品。
