EaStChem School of Chemistry, University of Edinburgh, Joseph Black Building, The King's Buildings, West Mains Rd, EH9 3JJ Edinburgh, UK.
Curr Opin Chem Biol. 2011 Dec;15(6):838-44. doi: 10.1016/j.cbpa.2011.10.004. Epub 2011 Nov 16.
Chromatin organization spans a wide range of structural complexity. Substructures at the 10-200nm scale are poorly characterized, especially in living cells, due to the limitations of electron microscopy and standard optical microscopy. Recently developed super-resolution fluorescence microscopy methods represent an exciting opportunity to access those substructures, and recent progress with these techniques has yielded insights into chromatin organization at different condensation stages. Recent studies have focused on confronting the challenges that are specific to chromatin super-resolution imaging, such as the high packing density of mitotic chromosomes and difficulties in interpreting interphase chromatin images. Building on these first results and with ongoing rapid technical advances in super-resolution fluorescence imaging there is great potential to uncover new features with unprecedented detail.
染色质组织跨越了广泛的结构复杂性范围。由于电子显微镜和标准光学显微镜的限制，10-200nm 尺度的亚结构特征描述较差，特别是在活细胞中。最近开发的超分辨率荧光显微镜方法为获取这些亚结构提供了一个令人兴奋的机会，并且这些技术的最新进展已经深入了解了不同浓缩阶段的染色质组织。最近的研究集中在解决特定于染色质超分辨率成像的挑战上，例如有丝分裂染色体的高密度包装和解释间期间染色质图像的困难。在这些初步结果的基础上，随着超分辨率荧光成像技术的快速发展，有很大的潜力以空前的细节揭示新的特征。
