University of Applied Sciences HTW Chur, Pulvermühlestrasse 57, 7004 Chur, Switzerland.
Institut für Physik, Universität Mainz, 55122 Mainz, Germany.
Genes (Basel). 2019 Jun 28;10(7):493. doi: 10.3390/genes10070493.
Since the advent of super-resolution microscopy, countless approaches and studies have been published contributing significantly to our understanding of cellular processes. With the aid of chromatin-specific fluorescence labeling techniques, we are gaining increasing insight into gene regulation and chromatin organization. Combined with super-resolution imaging and data analysis, these labeling techniques enable direct assessment not only of chromatin interactions but also of the function of specific chromatin conformational states.
自超分辨率显微镜问世以来,无数的方法和研究已经发表,这对我们理解细胞过程做出了重大贡献。借助于特异性荧光标记技术,我们对基因调控和染色质组织有了越来越深入的了解。这些标记技术与超分辨率成像和数据分析相结合,不仅能够直接评估染色质相互作用,还能够评估特定染色质构象状态的功能。