Haq Zulfqarul, Rastogi Ankur, Sharma Ramesh Kumar, Raghuwanshi Pratiksha, Singh Maninder, Khan Azmat Alam, Ahmad Syed Mudasir
Indian Council of Medical Research Project, Division of L.P.M, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, India.
Division of Animal Nutrition, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, India.
Vet Anim Sci. 2023 Dec 30;23:100331. doi: 10.1016/j.vas.2023.100331. eCollection 2024 Mar.
This study attempts to isolate a candidate growth promoter from the ovine paunch waste and scrutinize its effects on the production performance of broiler chickens as compared to mannan-oligosaccharide (MOS), a prebiotic, and lincomycin, an antibiotic growth promoter (AB). The paunch waste collected from slaughtered sheep was processed to remove particulate matter. The clarified liquid was then added to an excess of ethanol (1:9 ratio), and the resultant precipitate {(novel growth-promoting paunch extract (NGPE)} was collected, dried, and stored. increase in cell density for probiotic bacteria and (Log CFU/ml) were significantly higher ( < 0.01) in NGPE supplemented media (2.78 ± 0.11 and 2.77 ± 0.10) as compared to that on MOS (1.28 ± 0.05 and 2.49 ± 0.09) and glucose (1.09 ± 0.04 and 1.12 ± 0.04) supplemented media. In the in-vivo trial of six weeks duration with broiler chickens (Cobb-400), NGPE supplementation resulted in significantly higher growth in weeks IV ( < 0.05) and VI ( < 0.01) of age in comparison to MOS and AGP supplemented groups, a lower ( < 0.01) cumulative feed conversion ratio in comparison to MOS supplemented groups, and a higher ( < 0.01) cumulative protein efficiency ratio compared to MOS and AGP supplementation. NGPE supplementation also lowered lipid peroxidation ( < 0.01), increased reduced glutathione activity ( < 0.01) in chicken erythrocytes, and boosted the lactic acid bacteria count in the cecal contents ( < 0.01). This is the first report of the isolation of a paunch waste extract that increased the growth of probiotic bacteria and improved the production performance of broiler chickens.
本研究试图从绵羊瘤胃废弃物中分离出一种候选生长促进剂，并将其与益生元甘露寡糖（MOS）和抗生素生长促进剂（AB）林可霉素相比，仔细研究其对肉鸡生产性能的影响。从屠宰绵羊收集的瘤胃废弃物经过处理以去除颗粒物。然后将澄清液加入过量乙醇（1:9比例）中，收集所得沉淀物{新型生长促进瘤胃提取物（NGPE）}，干燥并储存。与补充MOS（1.28±0.05和2.49±0.09）和葡萄糖（1.09±0.04和1.12±0.04）的培养基相比，补充NGPE的培养基中益生菌的细胞密度增加和（Log CFU/ml）显著更高（P<0.01）。在对肉鸡（科宝400）进行的为期六周的体内试验中，与补充MOS和AGP的组相比，补充NGPE导致IV周龄（P<0.05）和VI周龄（P<0.01）时生长显著更高，与补充MOS的组相比，累积饲料转化率更低（P<0.01），与补充MOS和AGP相比，累积蛋白质效率更高（P<0.01）。补充NGPE还降低了脂质过氧化（P<0.01），增加了鸡红细胞中还原型谷胱甘肽活性（P<0.01），并提高了盲肠内容物中乳酸菌数量（P<0.01）。这是关于分离出一种能促进益生菌生长并提高肉鸡生产性能的瘤胃废弃物提取物的首次报道。
