Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Appl Environ Microbiol. 2012 Feb;78(3):876-7. doi: 10.1128/AEM.07039-11. Epub 2011 Nov 18.
Detection of environmental Burkholderia pseudomallei indicates a risk for melioidosis and is important for the development of a global risk map. We describe a simple method for detecting B. pseudomallei using direct culture of soil in enrichment broth. This gives a rate of positivity comparable to that obtained with a standard method but is cheaper and labor saving.
检测环境中的伯克霍尔德氏菌表明存在类鼻疽病的风险,对于绘制全球风险地图非常重要。我们描述了一种使用富集肉汤直接培养土壤来检测假鼻疽伯克霍尔德氏菌的简单方法。该方法的阳性检出率与标准方法相当,但成本更低,节省劳动力。