Instituto de Investigaciones Biotecnológicas, Instituto Tecnológico de Chascomús (IIB-INTECH) CONICET/UNSAM, Chascomús, Argentina.
Plant Signal Behav. 2011 Dec;6(12):1904-7. doi: 10.4161/psb.6.12.18050.
The transcriptomic response of A9:u-ATP9 and apetala3:u-ATP9 lines carrying a mitochondrial dysfunction in flower tissues has been characterized. Both lines showed an alteration in the transcription of several genes involved in carbon and nitrogen metabolism, stress responses, transcription factors and DNA binding proteins. Interestingly, several transcripts of photosynthetic-related genes were also affected in their expression such as the mRNAs encoding for chlorophyllase, chlorophyll binding proteins and a PSII. Moreover, chlorophyll levels were reduced and the Mg-dechelatase activity was increased, indicating an alteration in chlorophyll metabolism. Our results suggest that the mitochondrial dysfunction may also affect chloroplastic functions, and that our model could be useful to uncover retrograde signaling mechanisms operating between the three different plant genomes.
已经对携带线粒体功能障碍的 A9:u-ATP9 和 apetala3:u-ATP9 系在花组织中的转录组反应进行了表征。这两个系的几个涉及碳和氮代谢、应激反应、转录因子和 DNA 结合蛋白的基因的转录都发生了改变。有趣的是，几个与光合作用相关的基因的转录本的表达也受到了影响，例如编码叶绿素酶、叶绿素结合蛋白和 PSII 的 mRNA。此外，叶绿素水平降低，Mg-脱叶绿素酶活性增加，表明叶绿素代谢发生了改变。我们的结果表明，线粒体功能障碍也可能影响叶绿体的功能，并且我们的模型可能有助于揭示在三个不同的植物基因组之间起作用的逆行信号转导机制。
