Course of Health Sciences, Gunma University Graduate School of Health Sciences, Showa, Maebashi, Gunma, Japan.
Muscle Nerve. 2011 Dec;44(6):968-77. doi: 10.1002/mus.22224.
Community effect is a phenomenon caused by cell-cell communication during myogenesis. In myogenic C2C12 cells in vitro, the confluent phase is needed for myogenesis induction.
To examine the cell-density effect, growth kinetics and myogenic differentiation were investigated in cells plated at four different cell densities.
We found that expression of a myogenic differentiation marker was high in a density-dependent manner. At high density, where cell-cell contact was obvious, contact inhibition after the proliferation stage was accompanied by microarray findings demonstrating upregulation of negative regulating cell-cycle markers, including CDKI p21 and the muscle differentiation markers MyoD and myogenin. Interestingly, developmentally regulated protein expression (drebrin) protein expression was also upregulated in a density-dependent manner.
These results suggest that contact inhibition after the proliferation stage may induce growth arrest via cell-cell communication through the expression of CDKI p21 and may be responsible for progressing cell fusion.
群落效应是在肌生成过程中细胞间通讯引起的现象。在体外的成肌细胞 C2C12 中，需要达到细胞汇合阶段才能诱导肌生成。
为了研究细胞密度的影响，我们在四种不同的细胞密度下培养细胞，研究了细胞的生长动力学和肌生成分化。
我们发现肌生成分化标志物的表达呈密度依赖性增加。在高细胞密度下，细胞间接触明显，增殖阶段后的接触抑制伴随着微阵列研究结果，表明负调控细胞周期标志物，包括 CDKIs p21 和肌肉分化标志物 MyoD 和 myogenin 的上调。有趣的是，发育调节蛋白表达（drebrin）也呈密度依赖性上调。
这些结果表明，增殖阶段后的接触抑制可能通过 CDKI p21 的表达通过细胞间通讯诱导细胞生长停滞，并可能负责促进细胞融合。
