Sjollema J, van der Mei H C, Uyen H M, Busscher H J
Laboratory for Materia Technica, University of Groningen, The Netherlands.
FEMS Microbiol Lett. 1990 Jun 1;57(3):263-9. doi: 10.1016/0378-1097(90)90078-5.
The spatial arrangement of two strains of oral bacteria adhering on glass was studied in order to investigate cooperative effects in their adhesion mechanisms. Streptococcus salivarius HB was a strain which possessed several classes of fibrillar surface appendages, whereas on the cell surface of S. mutants NS no surface appendages could be identified. The bacteria were deposited from a flowing suspension with various buffer concentrations on the bottom glass plate of a parallel plate flow cell and were observed directly with a video camera mounted on a phase contrast microscope. The positions of all adhering bacteria were determined by means of automated real time image analysis and subsequently employed for calculating radial and angular pair distribution functions. Pair distribution functions indicate the average relative number density of bacteria around one deposited bacterium as a function of the radial distance or the angular orientation relative to the flow direction. From the calculated pair distribution functions of both bacterial strains it was concluded that cooperative effects contributed to the adhesion of S. salivarius HB, but not to adhesion of S. mutants NS. It was suggested that these cooperative effects originate from the surface appendages of S. salivarius HB.
为了研究两种口腔细菌在玻璃上附着的空间排列，以探究它们附着机制中的协同效应。唾液链球菌HB是一种具有几类纤维状表面附属物的菌株，而变异链球菌NS的细胞表面未发现表面附属物。将细菌从含有不同缓冲液浓度的流动悬浮液中沉积到平行平板流动池的底部玻璃板上，并用安装在相差显微镜上的摄像机直接观察。通过自动实时图像分析确定所有附着细菌的位置，随后用于计算径向和角向对分布函数。对分布函数表示围绕一个沉积细菌的细菌平均相对数密度，它是相对于流动方向的径向距离或角向取向的函数。从两种细菌菌株计算出的对分布函数得出结论，协同效应有助于唾液链球菌HB的附着，但对变异链球菌NS的附着没有作用。有人认为这些协同效应源自唾液链球菌HB的表面附属物。
