Panyutich A V, Prassolov V S, Shydlovskaya E A, Reznikov M V, Chumakov P M, Voitenok N N
Byelorussian Institute of Hematology and Blood Transfusion, Minsk, USSR.
Hybridoma. 1990 Aug;9(4):401-6. doi: 10.1089/hyb.1990.9.401.
We studied the possibility of syngeneic cells expressing heterologous protein being used for sensitization of mice and production of hybridomas. Recombinant retroviral vector containing cloned human somatotropic hormone (hSTH) gene was used to express hSTH in BALB/3T3 cells. BALB/c mice were injected intrasplenically (i/s) or combination of intraperitoneally (i/p) and intrasplenically with hSTH-producing cells. Sensitized splenocytes were fused with myeloma cell P3X63-AgB.653. Screening for anti-hSTH hybridomas was performed by enzyme-linked immunoassay. Both single i/s injection of producer cells as well as combined i/s and i/p injections were effective for sensitization of splenocytes. Combined injection was effective for production of IgG and IgM secreting hybridomas. Single i/s injection led to generation of only IgM producing hybridomas. The results proved that syngeneic cells expressing genes of heterologous proteins can be used for splenocyte sensitization and hybridoma preparation.
我们研究了表达异源蛋白的同基因细胞用于小鼠致敏和杂交瘤产生的可能性。使用含有克隆的人生长激素（hSTH）基因的重组逆转录病毒载体在BALB/3T3细胞中表达hSTH。将BALB/c小鼠经脾内（i/s）注射或腹腔内（i/p）与经脾内联合注射产生hSTH的细胞。将致敏的脾细胞与骨髓瘤细胞P3X63-AgB.653融合。通过酶联免疫测定法筛选抗hSTH杂交瘤。单独经脾内注射产生细胞以及经脾内和腹腔内联合注射对脾细胞致敏均有效。联合注射对产生分泌IgG和IgM的杂交瘤有效。单独经脾内注射仅导致产生分泌IgM的杂交瘤。结果证明，表达异源蛋白基因的同基因细胞可用于脾细胞致敏和杂交瘤制备。
