Barry M A, Barry M E, Johnston S A
University of Texas Southwestern Medical Center, Dallas.
Biotechniques. 1994 Apr;16(4):616-8, 620.
Genetic immunization is a simple method for producing polyclonal antibodies in mice. To test if this approach could be used for monoclonal antibody production, biolistic transfection was used to immunize a mouse. High levels of polyclonal antibodies against human growth hormone (hGH) were elicited following three inoculations with the gene for hGH. When hybridoma cells were created from the mouse's splenocytes, approximately 17% secreted antibodies vs. hGH. Of these, some recognized only native or denatured hGH, while most recognized both forms of the protein. These findings demonstrate the utility of genetic immunization as a method to produce monoclonal antibodies.
基因免疫是在小鼠体内产生多克隆抗体的一种简单方法。为了测试这种方法是否可用于单克隆抗体的生产，采用生物弹道转染法对一只小鼠进行免疫。用人生长激素（hGH）基因进行三次接种后，引发了针对hGH的高水平多克隆抗体。当从小鼠脾细胞中制备杂交瘤细胞时，约17%的细胞分泌抗hGH抗体。其中，一些细胞只识别天然或变性的hGH，而大多数细胞则识别这两种形式的蛋白质。这些发现证明了基因免疫作为一种生产单克隆抗体方法的实用性。
