Department of Chemistry, Zhengzhou University, Zhengzhou, 450001, P R China.
Analyst. 2012 Jan 21;137(2):414-9. doi: 10.1039/c1an15720e. Epub 2011 Nov 23.
A novel voltammetric sensor, based on DNA immobilized on the surface of an ethylenediamine/polyglutamic (En/PGA) modified glassy carbon electrode (GCE), was constructed and used for determination of dihydromyricetin (DMY). The electrochemical behaviour of DMY at this sensor was investigated in pH 3.6 NaAc-HAc buffer solutions by cyclic voltammetry (CV) and differential pulse anodic voltammetry (DPV). The oxidation of DMY is an adsorption-controlled irreversible process. The oxidation mechanism was proposed and discussed. It was found that the modified electrode exhibited a linear voltammetric response for DMY in the range of 4.0 × 10(-8) mol L(-1) to 2 × 10(-6) mol L(-1), with a detection limit of 2 × 10(-8) mol L(-1). The method was also applied successfully to detect DMY in an ampelopsis sample with satisfactory results.
一种基于 DNA 固定在乙二胺/多聚谷氨酸（En/PGA）修饰玻碳电极（GCE）表面的新型伏安传感器被构建并用于测定二氢杨梅素（DMY）。通过循环伏安法（CV）和差分脉冲阳极伏安法（DPV）在 pH 3.6 NaAc-HAc 缓冲溶液中研究了 DMY 在该传感器上的电化学行为。DMY 的氧化是一个受吸附控制的不可逆过程。提出并讨论了氧化机制。结果发现，修饰电极对 DMY 在 4.0×10(-8)mol L(-1)至 2.0×10(-6)mol L(-1)的范围内表现出线性伏安响应，检测限为 2.0×10(-8)mol L(-1)。该方法还成功地应用于检测蛇葡萄样品中的 DMY，结果令人满意。
