Si Xiaojing, Huang Yue, Han Mei, Luo Liqiang
Department of Food Science, Shanghai Business School, Shanghai 200235, China.
College of Sciences, Shanghai University, Shanghai 200444, China.
Molecules. 2025 Jan 5;30(1):180. doi: 10.3390/molecules30010180.
Dihydromyricetin (DMY), as the main active ingredient in , is a naturally occurring flavonoid that has attracted extensive attention for its multiple biological activities. For the quick and accurate measurement of DMY, a novel electrochemical sensor based on a glassy carbon electrode (GCE) modified with a cobalt metal-organic framework (Co-MOF) was proposed in this work. The Co-MOF was synthesized via a single-step hydrothermal process using Co(NO)·6HO. Fourier infrared spectroscopy, X-ray photoelectron spectroscopy and scanning electron microscopy were used to study the morphology and structure of the synthesized Co-MOF. Utilizing differential pulse voltammetry and cyclic voltammetry methods, the effectiveness of DMY electro-oxidation on the Co-MOF/GCE was examined. The results showed that, in comparison to the bare GCE, the electro-oxidation peak current of DMY was considerably increased by the Co-MOF/GCE. The detection limit was 0.07 μM, and the peak current demonstrated two linear relationships in the ranges of 0.2-20 μM and 20-100 μM, with the linear equations of Ip (μA) = 0.4729c (μM) + 1.0822 (R = 0.9913) and Ip (μA) = 0.0939c (μM) + 8.4178 (R = 0.9971), respectively. The average DMY content in samples was measured to be 3.275 μM, with a good recovery of 108.27% and a relative standard deviation value of 3.46%. The proposed method is simple, rapid and sensitive and can be used for the determination of DMY in .
二氢杨梅素（DMY）作为[具体物质]中的主要活性成分，是一种天然存在的黄酮类化合物，因其多种生物活性而受到广泛关注。为了快速准确地测定DMY，本文提出了一种基于钴金属有机框架（Co-MOF）修饰的玻碳电极（GCE）的新型电化学传感器。Co-MOF通过使用Co(NO)·6HO的一步水热法合成。采用傅里叶红外光谱、X射线光电子能谱和扫描电子显微镜研究了合成的Co-MOF的形貌和结构。利用差分脉冲伏安法和循环伏安法研究了DMY在Co-MOF/GCE上的电氧化效果。结果表明，与裸GCE相比，Co-MOF/GCE使DMY的电氧化峰电流显著增加。检测限为0.07 μM，峰电流在0.2 - 20 μM和20 - 100 μM范围内呈现两个线性关系，线性方程分别为Ip（μA） = 0.4729c（μM） + 1.0822（R = 0.9913）和Ip（μA） = 0.0939c（μM） + 8.4178（R = 0.9971）。测得[具体样品]中DMY的平均含量为3.275 μM，回收率良好，为108.27%，相对标准偏差值为3.46%。所提出的方法简单、快速且灵敏，可用于[具体样品]中DMY的测定。
