Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Mol Syst Biol. 2011 Nov 22;7:550. doi: 10.1038/msb.2011.84.
Assessing relevant molecular differences between human-induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) is important, given that such differences may impact their potential therapeutic use. Controversy surrounds recent gene expression studies comparing hiPSCs and hESCs. Here, we present an in-depth quantitative mass spectrometry-based analysis of hESCs, two different hiPSCs and their precursor fibroblast cell lines. Our comparisons confirmed the high similarity of hESCs and hiPSCS at the proteome level as 97.8% of the proteins were found unchanged. Nevertheless, a small group of 58 proteins, mainly related to metabolism, antigen processing and cell adhesion, was found significantly differentially expressed between hiPSCs and hESCs. A comparison of the regulated proteins with previously published transcriptomic studies showed a low overlap, highlighting the emerging notion that differences between both pluripotent cell lines rather reflect experimental conditions than a recurrent molecular signature.
评估人类诱导多能干细胞（hiPSCs）和人类胚胎干细胞（hESCs）之间的相关分子差异非常重要，因为这些差异可能会影响它们在治疗中的潜在应用。最近的基因表达研究比较了 hiPSCs 和 hESCs，引起了争议。在这里，我们对 hESCs、两种不同的 hiPSCs 及其前体细胞系进行了深入的定量质谱分析。我们的比较证实了 hESCs 和 hiPSCs 在蛋白质组水平上的高度相似性，因为 97.8%的蛋白质未发生变化。然而，一小部分 58 种蛋白质，主要与代谢、抗原处理和细胞黏附有关，在 hiPSCs 和 hESCs 之间的表达存在显著差异。与先前发表的转录组学研究比较调控蛋白表明，两者之间的重叠率较低，这突显了一个新的观点，即这两种多能细胞系之间的差异更多地反映了实验条件，而不是反复出现的分子特征。
