National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Biotechnol Lett. 2012 Mar;34(3):563-9. doi: 10.1007/s10529-011-0804-4. Epub 2011 Nov 23.
A hairy root culture system of Calotropis gigantea was established and effects of mechanical wounding (MW) and elicitors [methyl jasmonate (MJ), yeast extract (YE) and chitosan (CS)] on cardenolide production were investigated. All treatments stimulated the production of cardenolide in hairy root cultures of C. gigantea. CS was the most effective elicitor, followed by MJ. YE and MW also improved cardenolide yield in individual treatments. The highest cardenolide yield (1,050 ± 55 mg/l) was obtained after adding 50 mg CS/l for 20 days, which was 2.7-fold higher than the control.
建立了大猪屎豆（Calotropis gigantea）的发根培养体系，并研究了机械损伤（MW）和诱导剂[茉莉酸甲酯（MJ）、酵母提取物（YE）和壳聚糖（CS）]对强心苷产量的影响。所有处理均刺激大猪屎豆发根培养物中强心苷的产生。CS 是最有效的诱导剂，其次是 MJ。YE 和 MW 也分别提高了强心苷的产量。添加 50 mg CS/l 并培养 20 天后，获得了最高的强心苷产量（1050±55 mg/l），比对照提高了 2.7 倍。
