Medicinal Plant Laboratory, Department of Botany, Bose Institute, 93/1 APC Road, Kolkata 700009, India.
J Biosci Bioeng. 2011 Jun;111(6):706-10. doi: 10.1016/j.jbiosc.2011.02.003. Epub 2011 Mar 5.
Elicitation of Plumbago indica hairy roots with yeast carbohydrate fraction, chitosan, manganese chloride, copper chloride and methyl jasmonate exhibited significant elevation (~1.2 to 2 fold) of plumbagin production in shake flask culture as compared with control. Chitosan and methyl jasmonate elicitation also caused simultaneous plumbagin leaching into culture media. Three days' exposure of chitosan (200 mg l(-1)) and methyl jasmonate (80 μM) together synergized total plumbagin yield to its maximum 11.96 ± 0.76 mg g(-l) DW in shake flask culture. In bioreactor cultivation, a significant raise in fresh root biomass was recorded on day 20 as compared with control shake flask culture. Three days' exposure of chitosan (200 mg l(-1)) and methyl jasmonate (80 μM) with 20 days old bioreactor-culture significantly improved total plumbagin production to 13.16 ± 1.72 mg g(-l) DW with simultaneous plumbagin leaching into bioreactor media.
与对照相比，用酵母碳水化合物部分、壳聚糖、氯化锰、氯化铜和茉莉酸甲酯诱导印度板蓝的发根可使摇瓶培养中的白花丹素产量显著提高（~1.2 至 2 倍）。壳聚糖和茉莉酸甲酯的诱导也会导致白花丹素同时浸出到培养基中。在摇瓶培养中，壳聚糖（200mg/L）和茉莉酸甲酯（80μM）共同作用 3 天，协同作用将总白花丹素产量提高到最大值 11.96±0.76mg/g DW。在生物反应器培养中，与对照摇瓶培养相比，第 20 天记录到新鲜根生物量显著增加。壳聚糖（200mg/L）和茉莉酸甲酯（80μM）共同作用 3 天，同时用 20 天的生物反应器培养，可显著提高总白花丹素的产量，达到 13.16±1.72mg/g DW，同时将白花丹素浸出到生物反应器培养基中。
