Kurhanewicz J, Winguth S D, Char D H, Kaleta S, Kindy-Degnan N, Swift P S, Stauffer P R, Wendland M F, Lovato A, Chang L H
Department of Pharmaceutical Chemistry, University of California, San Francisco 94143.
Invest Ophthalmol Vis Sci. 1990 Sep;31(9):1745-53.
Scleral surface coils were used to obtain in vivo magnetic resonance spectra (MRS) of Greene melanoma implanted in the rabbit uvea. Well-localized tumor spectra (4.7 Tesla) with good signal-to-noise ratios (S/N) were obtained from the tumor with a "single-pulse" sequence in less than 1 hour. Tumor localization was confirmed with one-dimensional spectroscopic imaging studies. Serial 31P spectra were obtained during tumor growth and after both optimal and suboptimal hyperthermia. Early 31P MRS change is correlated with tumor treatment response and preceded histologic evidence of cell destruction. Twenty-four to 48 hours after successful treatment, the inorganic phosphate/nucleoside triphosphate (NTP), and phosphomonoester/NTP ratios were significantly increased from 1.2 +/- 0.1 to 1.7 +/- 0.1 and 1.3 +/- 0.1 to 1.8 +/- 0.2, respectively. In contrast, untreated or ineffectively treated tumors showed little change. Interpretation of 31P MRS data in this animal uveal melanoma model after the first week was complicated by decreased S/N, increased contamination from contiguous tissues, ingrowth of fibroblasts, macrophages, and intratumor hemorrhage.
使用巩膜表面线圈获取植入兔葡萄膜的格林氏黑色素瘤的体内磁共振波谱（MRS）。采用“单脉冲”序列，在不到1小时的时间内从肿瘤获得了定位良好、信噪比（S/N）高的肿瘤波谱（4.7特斯拉）。通过一维波谱成像研究确认了肿瘤定位。在肿瘤生长期间以及最佳和次最佳热疗后获取了连续的31P波谱。早期31P MRS变化与肿瘤治疗反应相关，且先于细胞破坏的组织学证据出现。成功治疗后24至48小时，无机磷酸盐/核苷三磷酸（NTP）以及磷酸单酯/NTP比值分别从1.2±0.1显著增加至1.7±0.1和从1.3±0.1显著增加至1.8±0.2。相比之下，未治疗或治疗无效的肿瘤变化很小。在该动物葡萄膜黑色素瘤模型中，第一周后31P MRS数据的解读因信噪比降低、邻近组织污染增加、成纤维细胞和巨噬细胞长入以及肿瘤内出血而变得复杂。
