Department of Pathology, Josephine Nefkens Institute, Erasmus MC, Rotterdam, The Netherlands.
PLoS One. 2011;6(11):e27704. doi: 10.1371/journal.pone.0027704. Epub 2011 Nov 16.
Within SPIDIA, an EC FP7 project aimed to improve pre analytic procedures, the PAXgene Tissue System (PAXgene), was designed to improve tissue quality for parallel molecular and morphological analysis. Within the SPIDIA project promising results were found in both genomic and proteomic experiments with PAXgene-fixed and paraffin embedded tissue derived biomolecules. But, for this technology to be accepted for use in both clinical and basic research, it is essential that its adequacy for preserving morphology and antigenicity is validated relative to formalin fixation. It is our aim to assess the suitability of PAXgene tissue fixation for (immuno)histological methods. Normal human tissue specimens (n = 70) were collected and divided into equal parts for fixation either with formalin or PAXgene. Sections of the obtained paraffin-embedded tissue were cut and stained. Morphological aspects of PAXgene-fixed tissue were described and also scored relative to formalin-fixed tissue. Performance of PAXgene-fixed tissue in immunohistochemical and in situ hybridization assays was also assessed relative to the corresponding formalin-fixed tissues. Morphology of PAXgene-fixed paraffin embedded tissue was well preserved and deemed adequate for diagnostics in most cases. Some antigens in PAXgene-fixed and paraffin embedded sections were detectable without the need for antigen retrieval, while others were detected using standard, formalin fixation based, immunohistochemistry protocols. Comparable results were obtained with in situ hybridization and histochemical stains. Basically all assessed histological techniques were found to be applicable to PAXgene-fixed and paraffin embedded tissue. In general results obtained with PAXgene-fixed tissue are comparable to those of formalin-fixed tissue. Compromises made in morphology can be called minor compared to the advantages in the molecular pathology possibilities.
在 SPIDIA 项目中，一个欧盟第七框架计划的项目，旨在改善分析前程序，PAXgene 组织系统（PAXgene）被设计用来提高组织质量，以进行平行的分子和形态学分析。在 SPIDIA 项目中，使用 PAXgene 固定和石蜡包埋组织衍生生物分子进行基因组和蛋白质组学实验都取得了有前景的结果。但是，为了使这项技术在临床和基础研究中得到接受，必须验证其相对于福尔马林固定的保留形态和抗原性的充分性。我们的目的是评估 PAXgene 组织固定用于（免疫）组织化学方法的适宜性。收集了 70 例正常人类组织标本，并将其等分为福尔马林或 PAXgene 固定组。获得的石蜡包埋组织的切片进行了切割和染色。描述了 PAXgene 固定组织的形态学方面，并相对于福尔马林固定组织进行了评分。还相对于相应的福尔马林固定组织评估了 PAXgene 固定组织在免疫组织化学和原位杂交测定中的性能。PAXgene 固定的石蜡包埋组织的形态学得到了很好的保存，在大多数情况下被认为足以用于诊断。在 PAXgene 固定和石蜡包埋切片中可以检测到一些抗原，而无需进行抗原修复，而其他抗原则使用标准的基于福尔马林固定的免疫组织化学方案进行检测。原位杂交和组织化学染色也获得了类似的结果。基本上所有评估的组织学技术都被发现适用于 PAXgene 固定和石蜡包埋组织。一般来说，PAXgene 固定组织获得的结果与福尔马林固定组织的结果相当。与分子病理学可能性相比，形态学上的妥协可以说是次要的。
