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使用多光谱漫散光测量对非球形颗粒进行形态层析成像。

Morphologic tomography of nonspherical particles using multispectral diffusing light measurements.

机构信息

University of Florida, Department of Biomedical Engineering, Gainesville, Florida 32611-6131, USA.

出版信息

J Biomed Opt. 2011 Nov;16(11):116014. doi: 10.1117/1.3650313.

DOI:10.1117/1.3650313
PMID:22112119
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3223512/
Abstract

A series of phantom experiments are conducted to demonstrate the ability of a T-matrix-based inverse algorithm for tomographic recovery of morphologic characteristics of nonspherical particles embedded in heterogeneous turbid media. Diffusely scattered light at several wavelengths along the boundary of the phantom are collected and analyzed to allow for simultaneous extraction of the size, concentration, and aspect ratio of the spheroidal particles.

摘要

进行了一系列的幽灵实验，以展示基于 T 矩阵的逆算法在层析恢复嵌入非球形粒子的异质混浊介质中的形态特征的能力。在幽灵的边界上沿几个波长收集和分析漫散射光，以允许同时提取球形粒子的大小、浓度和纵横比。

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Appl Opt. 2011 Jul 20;50(21):3896-906. doi: 10.1364/AO.50.003896.
2
Noninvasive identification of subcellular organization and nuclear morphology features associated with leukemic cells using light-scattering spectroscopy.利用光散射光谱技术无创识别与白血病细胞相关的亚细胞结构和核形态特征。
J Biomed Opt. 2011 Mar;16(3):037007. doi: 10.1117/1.3562925.
3
Size and shape determination of spheroidal scatterers using two-dimensional angle resolved scattering.利用二维角分辨散射确定球形散射体的尺寸和形状
Opt Express. 2010 Jul 5;18(14):14616-26. doi: 10.1364/OE.18.014616.
4
Imaging of particle size and concentration in heterogeneous turbid media with multispectral diffuse optical tomography.基于多光谱扩散光学层析成像技术对非均匀浑浊介质中颗粒大小和浓度的成像
Opt Express. 2004 Dec 13;12(25):6313-8. doi: 10.1364/opex.12.006313.
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Experimental verification of T-matrix-based inverse light scattering analysis for assessing structure of spheroids as models of cell nuclei.基于T矩阵的逆光散射分析用于评估作为细胞核模型的球体结构的实验验证。
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