Bhalla Sumati, Tandon Shobha, Satyamoorthy K
Department of Pedodontics and Preventive Dentistry, Manipal College of Dental Sciences, Manipal, India.
Contemp Clin Dent. 2010 Jan;1(1):17-22. doi: 10.4103/0976-237X.62515.
Early childhood caries (ECC) is a common disease process that afflicts a large proportion of the child population worldwide. Extensive research in past indicates that it is the result of bacterial infection, also influenced by host and dietary factors. Current caries research seeks to identify risk factors as well as natural oral defenses that may protect against or prevent caries development. Saliva, in spite of being the strongest defense system, still has a wide array of properties and proteins whose role is yet not clearly known.
To compare the resting human whole salivary characteristics in children with ECC and those who are caries free.
The study was conducted over a period of 9 months in 4- to 6-year-old 100 children comprising two groups - 50 with ECC and 50 caries free.
The whole salivary flow rate, pH, mean protein concentration, and the electrophoretic profile of salivary proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were compared among both groups.
The SPSS (version 11.0) software package was used to conduct the chi-square, Fisher's exact and Pearson's chi-square tests to compare the data.
On gel electrophoresis, there was a significant difference among both groups with caries-free subjects having a higher number of proline-rich protein bands, substantiating the protective role of this protein. A significantly higher number of glycoprotein bands were observed in the whole saliva of subjects with ECC. A significant inverse correlation between the mean protein concentration and the whole salivary flow rate was observed in both groups.
幼儿龋齿（ECC）是一种常见的疾病过程，困扰着全球很大一部分儿童群体。过去的大量研究表明，它是细菌感染的结果，也受宿主和饮食因素的影响。当前的龋齿研究旨在确定可能预防或防止龋齿发展的风险因素以及天然口腔防御机制。唾液尽管是最强的防御系统，但仍有大量特性和蛋白质，其作用尚不清楚。
比较患ECC儿童和无龋儿童的静息人全唾液特征。
该研究在4至6岁的100名儿童中进行，为期9个月，分为两组——50名患ECC儿童和50名无龋儿童。
比较两组的全唾液流速､pH值､平均蛋白质浓度以及通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）得到的唾液蛋白质电泳图谱。
使用SPSS（版本11.0）软件包进行卡方检验､Fisher精确检验和Pearson卡方检验以比较数据。
在凝胶电泳中，两组之间存在显著差异，无龋受试者富含脯氨酸的蛋白带数量更多，证实了该蛋白的保护作用。在患ECC受试者的全唾液中观察到糖蛋白带数量显著更多。两组中均观察到平均蛋白质浓度与全唾液流速之间存在显著负相关。
