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1
Synthesis of the iron-molybdenum cofactor of nitrogenase is inhibited by a low-molecular-weight metabolite of Klebsiella pneumoniae.肺炎克雷伯菌的一种低分子量代谢产物会抑制固氮酶铁钼辅因子的合成。
J Bacteriol. 1990 Oct;172(10):6084-9. doi: 10.1128/jb.172.10.6084-6089.1990.
2
Genes required for formation of the apoMoFe protein of Klebsiella pneumoniae nitrogenase in Escherichia coli.肺炎克雷伯菌固氮酶的载脂蛋白钼铁蛋白在大肠杆菌中形成所需的基因。
J Biol Chem. 1990 Sep 15;265(26):15909-19.
3
nifV-dependent, low-molecular-weight factor required for in vitro synthesis of iron-molybdenum cofactor of nitrogenase.固氮酶铁钼辅因子体外合成所需的依赖nifV的低分子量因子。
J Bacteriol. 1986 Sep;167(3):999-1003. doi: 10.1128/jb.167.3.999-1003.1986.
4
Biosynthesis of the iron-molybdenum cofactor of nitrogenase.固氮酶铁钼辅因子的生物合成
Crit Rev Biotechnol. 1994;14(3):225-49. doi: 10.3109/07388554409079834.
5
Electrophoretic studies on the assembly of the nitrogenase molybdenum-iron protein from the Klebsiella pneumoniae nifD and nifK gene products.关于肺炎克雷伯氏菌固氮酶钼铁蛋白由nifD和nifK基因产物组装的电泳研究。
J Biol Chem. 1992 Nov 25;267(33):24007-16.
6
Autoregulation of nitrogenase expression in Klebsiella pneumoniae.肺炎克雷伯菌中固氮酶表达的自动调节。
Microbiology (Reading). 1994 Aug;140 ( Pt 8):1917-25. doi: 10.1099/13500872-140-8-1917.
7
In vitro synthesis of the iron-molybdenum cofactor of nitrogenase. Purification and characterization of NifB cofactor, the product of NIFB protein.固氮酶铁钼辅因子的体外合成。NifB辅因子（NIFB蛋白的产物）的纯化与特性分析。
J Biol Chem. 1994 Jan 14;269(2):1154-8.
8
The nifH gene product is required for the synthesis or stability of the iron-molybdenum cofactor of nitrogenase from Klebsiella pneumoniae.肺炎克雷伯菌固氮酶的铁钼辅因子的合成或稳定性需要nifH基因产物。
Eur J Biochem. 1986 Oct 15;160(2):371-7. doi: 10.1111/j.1432-1033.1986.tb09981.x.
9
Involvement of GroEL in nif gene regulation and nitrogenase assembly.GroEL参与固氮基因调控和固氮酶组装。
J Bacteriol. 1991 Oct;173(20):6339-46. doi: 10.1128/jb.173.20.6339-6346.1991.
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In vitro synthesis of the iron-molybdenum cofactor of nitrogenase.固氮酶铁钼辅因子的体外合成。
Proc Natl Acad Sci U S A. 1986 Mar;83(6):1636-40. doi: 10.1073/pnas.83.6.1636.

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Nitrogen Fixation by Azotobacter vinelandii Strains Having Deletions in Structural Genes for Nitrogenase.维涅兰德固氮菌结构基因缺失的菌株的固氮作用。
Science. 1986 Apr 4;232(4746):92-4. doi: 10.1126/science.232.4746.92.
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Regulation of nitrogen fixation in Klebsiella pneumoniae: isolation and characterization of strains with nif-lac fusions.肺炎克雷伯菌中固氮作用的调控：携带nif - lac融合基因菌株的分离与特性分析
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Biosynthesis of iron-molybdenum cofactor in the absence of nitrogenase.在缺乏固氮酶的情况下铁钼辅因子的生物合成。
J Bacteriol. 1984 Sep;159(3):888-93. doi: 10.1128/jb.159.3.888-893.1984.
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Role of the nifQ gene product in the incorporation of molybdenum into nitrogenase in Klebsiella pneumoniae.肺炎克雷伯菌中nifQ基因产物在钼掺入固氮酶过程中的作用。
J Bacteriol. 1984 Apr;158(1):187-94. doi: 10.1128/jb.158.1.187-194.1984.
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Nitrogenase from nifV mutants of Klebsiella pneumoniae contains an altered form of the iron-molybdenum cofactor.肺炎克雷伯氏菌nifV突变体的固氮酶含有一种铁钼辅因子的变体形式。
Biochem J. 1984 Jan 1;217(1):317-21. doi: 10.1042/bj2170317.
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Isolation of a molybdenum--iron cluster from nitrogenase.从固氮酶中分离出钼铁簇。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3438-40. doi: 10.1073/pnas.78.6.3438.
7
The nitrogenase system from Azotobacter: two-enzyme requirement for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis.来自固氮菌的固氮酶系统：N₂还原需要两种酶、ATP依赖性H₂生成以及ATP水解。
Proc Natl Acad Sci U S A. 1966 Sep;56(3):979-86. doi: 10.1073/pnas.56.3.979.
8
Nitrogenase. I. Repression and derepression of the iron-molybdenum and iron proteins of nitrogenase in Azotobacter vinelandii.固氮酶。I. 棕色固氮菌中固氮酶的铁钼蛋白和铁蛋白的阻遏与去阻遏
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9
Nitrogenase. IV. Simple method of purification to homogeneity of nitrogenase components from Azotobacter vinelandii.固氮酶。IV. 从棕色固氮菌中纯化固氮酶组分至均一性的简易方法。
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10
Nitrogenase. 3. Nitrogenaseless mutants of Azotobacter vinelandii: activities, cross-reactions and EPR spectra.固氮酶。3. 棕色固氮菌无固氮酶突变体：活性、交叉反应和电子顺磁共振光谱
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肺炎克雷伯菌的一种低分子量代谢产物会抑制固氮酶铁钼辅因子的合成。

Synthesis of the iron-molybdenum cofactor of nitrogenase is inhibited by a low-molecular-weight metabolite of Klebsiella pneumoniae.

作者信息

Downs D M, Ludden P W, Shah V K

机构信息

Department of Biochemistry, University of Wisconsin, Madison 53706.

出版信息

J Bacteriol. 1990 Oct;172(10):6084-9. doi: 10.1128/jb.172.10.6084-6089.1990.

DOI:10.1128/jb.172.10.6084-6089.1990
PMID:2211526
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC526933/
Abstract

The in vitro synthesis of the iron-molybdenum cofactor nitrogenase was inhibited by a low-molecular-weight factor. This inhibitory factor was present in the membrane extracts of wild-type and nif mutant strains of Klebsiella pneumoniae that were grown under conditions that either repressed or derepressed nitrogenase expression. In vitro, the inhibition was specific for the NifB protein. Addition of this factor to K. pneumoniae cells at various times during nif derepression decreased nitrogenase activity, presumably through inhibition of iron-molybdenum cofactor synthesis. The inhibitor was purified by solvent extraction and chromatography on DEAE-cellulose, silica gel, and aluminum oxide columns.

摘要

铁钼辅因子固氮酶的体外合成受到一种低分子量因子的抑制。这种抑制因子存在于肺炎克雷伯菌野生型和固氮酶突变株的膜提取物中，这些菌株在抑制或解除抑制固氮酶表达的条件下生长。在体外，这种抑制作用对NifB蛋白具有特异性。在固氮酶解除抑制的不同时间向肺炎克雷伯菌细胞中添加该因子会降低固氮酶活性，推测是通过抑制铁钼辅因子的合成。通过溶剂萃取以及在DEAE-纤维素、硅胶和氧化铝柱上进行色谱分离对该抑制剂进行了纯化。