MacNeil D, Zhu J, Brill W J
J Bacteriol. 1981 Jan;145(1):348-57. doi: 10.1128/jb.145.1.348-357.1981.
Strains with lac fused to each of the seven nif operons were isolated by two different methods. Repressing conditions prevented expression of all nif operons, whereas derepressing conditions led to the expression of all nif operons. No differences in Nif regulation were observed between Escherichia coli and Klebsiella pneumoniae with the same nif-lac fusions. Most derivatives of nif-lac fusion strains selected on lactose and NH4+ contained nif operator mutations. Some derivative contained deletions, which establishes that the direction of transcription of all seven nif operons is toward his
通过两种不同方法分离出了lac与七个nif操纵子中的每一个融合的菌株。抑制条件会阻止所有nif操纵子的表达，而解除抑制条件则会导致所有nif操纵子的表达。在具有相同nif-lac融合的大肠杆菌和肺炎克雷伯菌之间，未观察到Nif调控的差异。在乳糖和NH4+上选择的nif-lac融合菌株的大多数衍生物都含有nif操纵基因突变。一些衍生物含有缺失，这表明所有七个nif操纵子的转录方向都朝向his
