改良 Hodge 试验检测肠杆菌科中新型碳青霉烯酶的价值。
Value of the modified Hodge test for detection of emerging carbapenemases in Enterobacteriaceae.
机构信息
Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris Sud, K.-Bicêtre, France.
出版信息
J Clin Microbiol. 2012 Feb;50(2):477-9. doi: 10.1128/JCM.05247-11. Epub 2011 Nov 23.
Abstract
The modified Hodge test has an excellent sensitivity for detecting enterobacterial isolates producing Ambler class A (KPC) and class D (OXA-48) carbapenemases. Its sensitivity is low for NDM-1 producers (50%) but is increased to 85.7% by adding ZnSO(4) (100 μg/ml) in the culture medium. However, this test has a low specificity and is time-consuming.
摘要
改良 Hodge 试验对检测产 Ambler 类 A（KPC）和 D 类（OXA-48）碳青霉烯酶的肠杆菌具有极好的灵敏度。它对产 NDM-1 的细菌（50%）的灵敏度较低，但在培养基中添加 ZnSO(4)（100μg/ml）后可提高至 85.7%。然而，该试验特异性低且耗时。
相似文献
1
Value of the modified Hodge test for detection of emerging carbapenemases in Enterobacteriaceae.改良 Hodge 试验检测肠杆菌科中新型碳青霉烯酶的价值。
J Clin Microbiol. 2012 Feb;50(2):477-9. doi: 10.1128/JCM.05247-11. Epub 2011 Nov 23.
2
Use of ChromID extended-spectrum beta-lactamase medium for detecting carbapenemase-producing Enterobacteriaceae.使用 ChromID 扩展谱β-内酰胺酶培养基检测产碳青霉烯酶肠杆菌科。
J Clin Microbiol. 2010 May;48(5):1913-4. doi: 10.1128/JCM.02277-09. Epub 2010 Mar 17.
3
Rapid detection of OXA-48-like, KPC, NDM, and VIM carbapenemases in Enterobacterales by a new multiplex immunochromatographic test.新型多重免疫层析试验快速检测肠杆菌科中的 OXA-48 样、KPC、NDM 和 VIM 碳青霉烯酶。
Eur J Clin Microbiol Infect Dis. 2019 Feb;38(2):331-335. doi: 10.1007/s10096-018-3432-2. Epub 2018 Nov 17.
4
Evaluation of the Oxoid Brilliance™ CRE Agar for the detection of carbapenemase-producing Enterobacteriaceae.评估 Oxoid Brilliance™ CRE 琼脂对产碳青霉烯酶肠杆菌科的检测。
Eur J Clin Microbiol Infect Dis. 2013 Nov;32(11):1445-9. doi: 10.1007/s10096-013-1896-7. Epub 2013 Jun 2.
5
CHROMagar™ ESBL/mSuperCARBA bi-plate medium for detection of ESBL- and carbapenemase-producing Enterobacteriaceae from spiked stools.CHROMagar ESBL/mSuperCARBA 双板培养基用于检测粪便中添加的产 ESBL 和碳青霉烯酶肠杆菌科细菌。
Diagn Microbiol Infect Dis. 2019 Oct;95(2):107-112. doi: 10.1016/j.diagmicrobio.2019.05.002. Epub 2019 May 8.
6
Evaluation of Etest® strips for detection of KPC and metallo-carbapenemases in Enterobacteriaceae.检测肠杆菌科中产碳青霉烯酶和金属β-内酰胺酶的 Etest® 条的评估。
Diagn Microbiol Infect Dis. 2013 Nov;77(3):200-1. doi: 10.1016/j.diagmicrobio.2013.08.002. Epub 2013 Sep 14.
7
A disc diffusion assay for detection of class A, B and OXA-48 carbapenemases in Enterobacteriaceae using phenyl boronic acid, dipicolinic acid and temocillin.采用苯硼酸、二吡啶酸和替莫西林检测肠杆菌科中 A、B 和 OXA-48 碳青霉烯酶的纸片扩散法。
Clin Microbiol Infect. 2014 Apr;20(4):345-9. doi: 10.1111/1469-0691.12322. Epub 2013 Aug 9.
8
Detection of carbapenemase activity in Enterobacteriaceae using LC-MS/MS in comparison with the neo-rapid CARB kit using direct visual assessment and colorimetry.使用液相色谱-串联质谱法（LC-MS/MS）检测肠杆菌科细菌中的碳青霉烯酶活性，并与采用直接视觉评估和比色法的新型快速CARB试剂盒进行比较。
J Microbiol Methods. 2016 Dec;131:68-72. doi: 10.1016/j.mimet.2016.10.005. Epub 2016 Oct 11.
9
Rapid Identification of OXA-48-like, KPC, NDM, and VIM Carbapenemase-Producing Enterobacteriaceae From Culture: Evaluation of the RESIST-4 O.K.N.V. Multiplex Lateral Flow Assay.快速鉴定产 OXA-48 样、KPC、NDM 和 VIM 碳青霉烯酶肠杆菌科：RESIST-4 O.K.N.V. 多重侧向流检测的评估。
Ann Lab Med. 2020 May;40(3):259-263. doi: 10.3343/alm.2020.40.3.259.
10
Evaluation of two new commercial immunochromatographic assays for the rapid detection of OXA-48 and KPC carbapenemases from cultured bacteria.评估两种新型商用免疫层析检测法用于从培养细菌中快速检测OXA-48和KPC碳青霉烯酶。
J Antimicrob Chemother. 2016 May;71(5):1217-22. doi: 10.1093/jac/dkv472. Epub 2016 Jan 28.
引用本文的文献
1
High-throughput screening of monoclonal antibodies against carbapenemases using a multiplex protein microarray platform.使用多重蛋白质微阵列平台对碳青霉烯酶单克隆抗体进行高通量筛选。
Front Microbiol. 2025 Aug 26;16:1650094. doi: 10.3389/fmicb.2025.1650094. eCollection 2025.
2
Fundamental changes in the antimicrobial resistance profile of Klebsiella quasipneumoniae ATCC 700603 in response to meropenem stress.肺炎克雷伯菌ATCC 700603对美罗培南应激反应的抗菌药物耐药谱的根本变化。
BMC Microbiol. 2025 Jun 26;25(1):369. doi: 10.1186/s12866-025-04100-6.
3
Parallel Detection of the Unamplified Carbapenem Resistance Genes and Using a Plasmonic Nano-Biosensor with a Field-Portable DNA Extraction Method.使用具有现场便携式DNA提取方法的等离子体纳米生物传感器对未扩增的碳青霉烯耐药基因进行平行检测。
Biosensors (Basel). 2025 Feb 14;15(2):112. doi: 10.3390/bios15020112.
4
Phenotypic and molecular methods of carbapenemase detection: Can we break the chain and preserve the carbapenems?碳青霉烯酶检测的表型和分子方法：我们能否阻断传播链并保留碳青霉烯类药物？
Med J Armed Forces India. 2024 Nov-Dec;80(6):657-666. doi: 10.1016/j.mjafi.2023.05.008. Epub 2023 Jul 18.
5
Predictive Application Value of Metagenomic Next-Generation Sequencing in the Resistance of Carbapenem-Resistant Enterobacteriaceae.宏基因组下一代测序在耐碳青霉烯类肠杆菌科细菌耐药性中的预测应用价值
Can J Infect Dis Med Microbiol. 2025 Jan 6;2025:6619016. doi: 10.1155/cjid/6619016. eCollection 2025.
6
Laboratory detection of carbapenemases among Gram-negative organisms.革兰氏阴性菌中碳青霉烯酶的实验室检测
Clin Microbiol Rev. 2024 Dec 10;37(4):e0005422. doi: 10.1128/cmr.00054-22. Epub 2024 Nov 15.
7
Evaluation of point mutation carbapenemase-producing in Prapokklao Hospital, Thailand.泰国普拉波克拉奥医院产碳青霉烯酶点突变情况的评估。
Microbiol Spectr. 2024 Oct 17;12(12):e0019824. doi: 10.1128/spectrum.00198-24.
8
Detection of Carbapenem Resistance Genes by qPCR: Choosing the Right Method for Total DNA Extraction.通过定量聚合酶链反应检测碳青霉烯类耐药基因：选择正确的总DNA提取方法
Microorganisms. 2024 Jun 25;12(7):1285. doi: 10.3390/microorganisms12071285.
9
Carbapenemase producing at a large teaching hospital in Ohio: comparison to state surveillance and retrospective analysis of patient characteristics.俄亥俄州一家大型教学医院产碳青霉烯酶情况：与州监测数据的比较及患者特征回顾性分析
Infect Prev Pract. 2024 Apr 27;6(2):100366. doi: 10.1016/j.infpip.2024.100366. eCollection 2024 Jun.
10
The genomic characterization of carbapenem-resistant at a tertiary hospital in South Africa.南非一家三级医院耐碳青霉烯类细菌的基因组特征分析
JAC Antimicrob Resist. 2023 Jul 25;5(4):dlad089. doi: 10.1093/jacamr/dlad089. eCollection 2023 Aug.
本文引用的文献
1
Global spread of Carbapenemase-producing Enterobacteriaceae.产碳青霉烯酶肠杆菌科的全球传播。
Emerg Infect Dis. 2011 Oct;17(10):1791-8. doi: 10.3201/eid1710.110655.
2
Genetic features of blaNDM-1-positive Enterobacteriaceae.blaNDM-1 阳性肠杆菌科的遗传特征。
Antimicrob Agents Chemother. 2011 Nov;55(11):5403-7. doi: 10.1128/AAC.00585-11. Epub 2011 Aug 22.
3
Emergence of OXA-48-producing Escherichia coli clone ST38 in France.法国产OXA-48型大肠杆菌克隆株ST38的出现。
Antimicrob Agents Chemother. 2011 Oct;55(10):4937-8. doi: 10.1128/AAC.00413-11. Epub 2011 Jul 18.
4
Molecular analysis of NDM-1-producing enterobacterial isolates from Geneva, Switzerland.瑞士日内瓦产 NDM-1 的肠杆菌的分子分析。
J Antimicrob Chemother. 2011 Aug;66(8):1730-3. doi: 10.1093/jac/dkr174. Epub 2011 May 31.
5
Comparative evaluation of a chromogenic agar medium, the modified Hodge test, and a battery of meropenem-inhibitor discs for detection of carbapenemase activity in Enterobacteriaceae.比较显色琼脂培养基、改良 Hodge 试验和一系列美罗培南抑制剂纸片对肠杆菌科中产碳青霉烯酶活性的检测。
J Clin Microbiol. 2011 May;49(5):1965-9. doi: 10.1128/JCM.00203-11. Epub 2011 Mar 23.
6
Early dissemination of NDM-1- and OXA-181-producing Enterobacteriaceae in Indian hospitals: report from the SENTRY Antimicrobial Surveillance Program, 2006-2007.2006-2007 年 SENTRY 抗菌药物监测计划：印度医院中产生 NDM-1 和 OXA-181 的肠杆菌科细菌的早期传播。
Antimicrob Agents Chemother. 2011 Mar;55(3):1274-8. doi: 10.1128/AAC.01497-10. Epub 2010 Dec 28.
7
NDM-1-producing Escherichia coli in Germany.德国产NDM-1的大肠杆菌
Antimicrob Agents Chemother. 2011 Mar;55(3):1318-9. doi: 10.1128/AAC.01585-10. Epub 2010 Dec 28.
8
How to detect NDM-1 producers.如何检测 NDM-1 产生菌。
J Clin Microbiol. 2011 Feb;49(2):718-21. doi: 10.1128/JCM.01773-10. Epub 2010 Dec 1.
9
Emergence of metallo-β-lactamase NDM-1-producing multidrug-resistant Escherichia coli in Australia.澳大利亚出现产金属β-内酰胺酶 NDM-1 的多重耐药大肠杆菌。
Antimicrob Agents Chemother. 2010 Nov;54(11):4914-6. doi: 10.1128/AAC.00878-10. Epub 2010 Sep 7.
10
Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli.改良 Hodge 试验联合麦康凯琼脂用于筛选产碳青霉烯酶革兰阴性杆菌的性能改善。
J Microbiol Methods. 2010 Nov;83(2):149-52. doi: 10.1016/j.mimet.2010.08.010. Epub 2010 Aug 27.
Zotero 插件