Gu G, Zhang Z, Wang G, Han F, Han L, Wang K, Liu J, Li W
Department of Anaesthesiology, The Third Affiliated Hospital, Harbin Medical University, Harbin, China.
J Int Med Res. 2011;39(5):1783-97. doi: 10.1177/147323001103900521.
This study investigated the effect of electroacupuncture pretreatment on the lipopolysaccharide (LPS)-induced inflammatory response and on acute kidney injury in adult male pathogen-free Wistar rats. Rats received electroacupuncture at the Zusanli (ST36) and Neiguan (PC6) acupoints, or electrical stimulation at sham points, for 30 min before stimulation with either 5 mg/kg LPS intravenously or normal saline. Plasma cytokines, plasma nitrite, renal inducible nitric oxide synthase (iNOS) and nuclear factor κB (NF-κB) activity were assessed 240 min after LPS or normal saline injection. Blood urea nitrogen (BUN), creatinine (Cr) and histopathological score for renal tubular damage were also measured. Electroacupuncture pretreatment significantly decreased LPS-induced plasma tumour necrosis factor-α and interleukin (IL)-1β, increased plasma IL-10, and decreased plasma nitrite, renal iNOS and NF-κB activity. It also significantly decreased LPS-induced BUN, Cr and the renal histopathological score. These findings suggest that electroacupuncture pretreatment at the ST36 and PC6 acupoints attenuated the LPS-induced inflammatory response and mitigated acute kidney injury.
本研究调查了电针预处理对成年雄性无特定病原体Wistar大鼠脂多糖（LPS）诱导的炎症反应及急性肾损伤的影响。大鼠在静脉注射5mg/kg LPS或生理盐水刺激前30分钟，接受足三里（ST36）和内关（PC6）穴位的电针治疗，或假穴位的电刺激。在注射LPS或生理盐水240分钟后，评估血浆细胞因子、血浆亚硝酸盐、肾诱导型一氧化氮合酶（iNOS）和核因子κB（NF-κB）活性。还测量了血尿素氮（BUN）、肌酐（Cr）和肾小管损伤的组织病理学评分。电针预处理显著降低了LPS诱导的血浆肿瘤坏死因子-α和白细胞介素（IL）-1β，增加了血浆IL-10，并降低了血浆亚硝酸盐、肾iNOS和NF-κB活性。它还显著降低了LPS诱导的BUN、Cr和肾组织病理学评分。这些发现表明，ST36和PC6穴位的电针预处理减轻了LPS诱导的炎症反应并减轻了急性肾损伤。
