Department of Chemical Engineering, McGill University, Montreal, Quebec, Canada.
Colloids Surf B Biointerfaces. 2012 Mar 1;91:198-204. doi: 10.1016/j.colsurfb.2011.10.056. Epub 2011 Nov 6.
The use of engineered nanoparticles (ENPs) in commercial products has increased substantially over the last few years. Some research has been conducted in order to determine whether or not such materials are cytotoxic, but questions remain regarding the role that physiological media and sera constituents play in ENP aggregation or stabilization. In this study, several characterization methods were used to evaluate the particle size and surface potential of 6 ENPs suspended in a number of culture media and in the presence of different culture media constituents. Dynamic light scattering (DLS) and fluorescence correlation spectroscopy (FCS) were employed for size determinations. Results were interpreted on the basis of ENP surface potentials evaluated from particle electrophoretic mobilities (EPM). Measurements made after 24h of incubation at 37°C showed that the cell culture medium constituents had only moderate impact on the physicochemical properties of the ENP, although incubation in bovine serum albumin destabilized the colloidal system. In contrast, most of the serum proteins increased colloidal stabilization. Moreover, the type of ENP surface modification played a significant role in ENP behavior whereby the complexity of interactions between the ENPs and the medium components generally decreased with increasing complexity of the particle surface. This investigation emphasizes the importance of ENP characterization under conditions that are representative of cell culture media or physiological conditions for improved assessments of nanoparticle cytotoxicity.
在过去的几年中，工程纳米粒子（ENPs）在商业产品中的应用大大增加。已经进行了一些研究，以确定这些材料是否具有细胞毒性，但关于生理介质和血清成分在 ENP 聚集或稳定化中的作用仍存在疑问。在这项研究中，使用了几种表征方法来评估悬浮在多种培养基中和存在不同培养基成分中的 6 种 ENP 的粒径和表面电位。使用动态光散射（DLS）和荧光相关光谱（FCS）进行尺寸测定。根据从颗粒电泳迁移率（EPM）评估的 ENP 表面电位来解释结果。在 37°C 孵育 24 小时后的测量结果表明，细胞培养基成分对 ENP 的物理化学性质只有适度的影响，尽管牛血清白蛋白的孵育使胶体系统不稳定。相比之下，大多数血清蛋白增加了胶体稳定性。此外，ENP 表面改性的类型在 ENP 行为中起着重要作用，其中 ENP 与介质成分之间的相互作用的复杂性通常随着颗粒表面复杂性的增加而降低。这项研究强调了在代表细胞培养基或生理条件的条件下对 ENP 进行表征的重要性，以改善对纳米颗粒细胞毒性的评估。
