Virology Unit, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
Ecohealth. 2011 Dec;8(4):432-43. doi: 10.1007/s10393-011-0725-7. Epub 2011 Nov 29.
In order to evaluate the circulation of hantaviruses present in southeast Asia, a large scale survey of small mammal species was carried out at seven main sites in the region (Cambodia, Lao People's Democratic Republic, and Thailand). Small scale opportunistic trapping was also performed at an eighth site (Cambodia). Using a standard IFA test, IgG antibodies reacting to Hantaan virus antigens were detected at six sites. Antibody prevalence at each site varied from 0 to 5.6% with antibodies detected in several rodent species (Bandicota indica, B. savilei, Maxomys surifer, Mus caroli, M. cookii, Rattus exulans, R. nitidius, R. norvegicus, and R. tanezumi). When site seroprevalence was compared with site species richness, seropositive animals were found more frequently at sites with lower species richness. In order to confirm which hantavirus species were present, a subset of samples was also subjected to RT-PCR. Hantaviral RNA was detected at a single site from each country. Sequencing confirmed the presence of two hantavirus species, Thailand and Seoul viruses, including one sample (from Lao PDR) representing a highly divergent strain of Seoul virus. This is the first molecular evidence of hantavirus in Lao PDR and the first reported L segment sequence data for Thailand virus.
为了评估东南亚存在的汉坦病毒的传播情况，在该地区的七个主要地点（柬埔寨、老挝人民民主共和国和泰国）对小型哺乳动物物种进行了大规模调查。在第八个地点（柬埔寨）也进行了小规模的机会性诱捕。使用标准的IFA 检测，在六个地点检测到针对汉坦病毒抗原的 IgG 抗体。每个地点的抗体流行率从 0 到 5.6%不等，在几种啮齿动物（印度囊尾蚴、B. savilei、Maxomys surifer、Mus caroli、M. cookii、Rattus exulans、R. nitidius、R. norvegicus 和 R. tanezumi）中检测到抗体。当将地点血清阳性率与地点物种丰富度进行比较时，在物种丰富度较低的地点发现了更多的血清阳性动物。为了确认存在哪些汉坦病毒，还对部分样本进行了 RT-PCR。从每个国家的一个地点检测到汉坦病毒 RNA。测序证实存在两种汉坦病毒，即泰国病毒和首尔病毒，其中一个样本（来自老挝人民民主共和国）代表首尔病毒的高度分化株。这是老挝人民民主共和国首例汉坦病毒的分子证据，也是泰国病毒 L 段序列数据的首次报道。
