School of Marine Biomedical Sciences, Jeju National University, 1 Ara-dong, Jeju-si, Jeju 690-756, Korea.
Nutr Res Pract. 2012 Jun;6(3):187-94. doi: 10.4162/nrp.2012.6.3.187. Epub 2012 Jun 30.
In this study, the antibacterial effect was evaluated to determine the benefits of high speed drying (HSD) and far-infrared radiation drying (FIR) compared to the freeze drying (FD) method. Citrus press-cakes (CPCs) are released as a by-product in the citrus processing industry. Previous studies have shown that the HSD and FIR drying methods are much more economical for drying time and mass drying than those of FD, even though FD is the most qualified drying method. The disk diffusion assay was conducted, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined with methanol extracts of the dried CPCs against 11 fish and five food-related pathogenic bacteria. The disk diffusion results indicated that the CPCs dried by HSD, FIR, and FD prevented growth of all tested bacteria almost identically. The MIC and MBC results showed a range from 0.5-8.0 mg/mL and 1.0-16.0 mg/mL respectively. Scanning electron microscopy indicated that the extracts changed the morphology of the bacteria cell wall, leading to destruction. These results suggest that CPCs dried by HSD and FIR showed strong antibacterial activity against pathogenic bacteria and are more useful drying methods than that of the classic FD method in CPCs utilization.
在这项研究中，评估了抗菌效果，以确定高速干燥（HSD）和远红外辐射干燥（FIR）与冷冻干燥（FD）方法相比的优势。柑橘压榨饼（CPCs）是柑橘加工业的副产品。以前的研究表明，与 FD 相比，HSD 和 FIR 干燥方法在干燥时间和批量干燥方面更经济，尽管 FD 是最合格的干燥方法。进行了圆盘扩散试验，并使用甲醇提取物测定了干燥 CPCs 对 11 种鱼类和 5 种食源性致病菌的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。圆盘扩散结果表明，通过 HSD、FIR 和 FD 干燥的 CPCs 几乎完全阻止了所有测试细菌的生长。MIC 和 MBC 的结果分别显示范围为 0.5-8.0mg/mL 和 1.0-16.0mg/mL。扫描电子显微镜表明，提取物改变了细菌细胞壁的形态，导致破坏。这些结果表明，通过 HSD 和 FIR 干燥的 CPCs 对致病菌表现出强烈的抗菌活性，并且在 CPCs 的利用方面比经典的 FD 方法更有用。
